PerCP/Cyanine5.5 Rabbit anti-Human CD56/NCAM1 Monoclonal Antibody for FC - P13591

Core Product Specifications and Parameters

Biological Background: NCAM1/CD56 Function and Localization

• Neural cell adhesion molecule 1 (NCAM1), also designated CD56 and MSK39, is a multifunctional member of the immunoglobulin superfamily involved in cell-cell adhesion.
• NCAM1 plays pivotal roles in the nervous system, including neuron-neuron adhesion, neurite fasciculation, and neurite outgrowth during development and plasticity.
• The protein can act as a host cell receptor for neurotropic viruses such as rabies virus and Zika virus, mediating viral entry.
• Multiple protein isoforms are produced by alternative splicing, yielding transmembrane, GPI-anchored, and secreted forms localized to the cell membrane and extracellular space.
• Key post-translational modifications include N-linked glycosylation, disulfide bond formation, and phosphorylation, which regulate its adhesive properties and signaling.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 20-413) targets the extracellular region, covering the immunoglobulin-like domains; this design likely enables recognition of major NCAM1 isoforms, including GPI-anchored and transmembrane variants.
• For flow cytometry applications, titrate the antibody to determine the optimal working dilution for your specific cell population, and include appropriate isotype controls and viability dyes.
• Validate staining in known CD56-positive cells, such as NK cells or neuronal cell lines, and consider the potential impact of protease-mediated ectodomain shedding on surface detection.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we accelerate your assay development by providing a curated portfolio of IVD-grade antibodies and associated raw materials, including validated pairs and rare target sourcing. This anti-CD56/NCAM1 antibody serves neuroscience and virology research, and our supply chain ensures consistent quality and scalability. Partner with us to bridge your concept to clinic efficiently.