PerCP/Cyanine5.5 Rabbit anti-Human CD31/PECAM1 Monoclonal Antibody for FC - Q08481

Core Product Specifications and Parameters

Biological Background: PECAM1 Function and Localization

• PECAM1 (Platelet endothelial cell adhesion molecule), also known as CD31, is a member of the immunoglobulin superfamily of cell adhesion molecules.
• It plays a critical role in leukocyte transendothelial migration (TEM) under inflammatory conditions, with tyrosine phosphorylation at Tyr-679 being essential for trafficking.
• Homophilic trans-interaction of PECAM1 contributes to endothelial cell-cell adhesion at junctions, while heterophilic interaction with CD177 facilitates neutrophil transmigration.
• PECAM1 inhibits macrophage-mediated phagocytosis of viable leukocytes through a detachment signal but promotes clearance of apoptotic cells.
• It modulates bradykinin receptor signaling and regulates ERK1/2 activation in endothelial cells in response to hyperosmotic shock.
• PECAM1 has been implicated in susceptibility to atherosclerosis. Related references: PMID:19048083
• Subcellular localization includes the cell membrane, membrane rafts, and cell junctions. Post-translational modifications encompass palmitoylation, phosphorylation, and glycosylation.
• Tissue expression is detected in lung and platelets at the protein level.

Experimental Guidance and Technical Tips

• The recombinant immunogen covering the extracellular domain (aa 28-601) suggests that this antibody may be suited for flow cytometry applications targeting surface-expressed CD31; consider titrating to determine the optimal concentration.
• When developing flow cytometry assays, use appropriate isotype controls and validate in the intended sample matrix, accounting for CD31 expression on endothelial cells, platelets, and leukocyte subsets.
• Because CD31 is heavily glycosylated, verify that the antibody recognizes the native protein conformation under your experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers with streamlined access to high-quality IVD raw materials like this anti-CD31 monoclonal antibody, along with validated antibody pairs, bulk ancillary reagents, and rare-target sourcing. Our integrated supply chain and technical support bridge the gap from early development to commercialization, empowering your diagnostic workflow with reliable, research-use materials tailored to vascular and inflammation research.