PerCP/Cyanine5.5 Rabbit anti-Human CD27 Monoclonal Antibody for Flow Cytometry - P26842

Core Product Specifications and Parameters

Biological Background: CD27 Function and Localization

• CD27 is a costimulatory immune-checkpoint receptor of the tumor necrosis factor receptor superfamily, expressed on the surface of T-cells, NK-cells, and B-cells. Related references: PMID:28011863
• It binds to its ligand CD70 (CD27L) expressed by B-cells, mediating antigen-specific T-cell activation and expansion, thereby providing immune surveillance of B-cells. Related references: PMID:28011863
• Upon CD70 ligation, CD27 activates the TRAF2-PTPN6 axis to inhibit LCK phosphorylation, driving T-cell memory adaptations. Related references: PMID:38354704
• CD27 provides a negative feedback signal to leukocyte differentiation during immune activation, thus modulating hematopoiesis.
• In adipose tissue, CD27 negatively regulates the function of Th2 lymphocytes.
• CD27 is localized at the cell membrane and is found in most T-lymphocytes.
• The protein undergoes phosphorylation and glycosylation, contains disulfide bonds, and features a transmembrane helix; it is associated with apoptosis signaling.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 21–192), making this antibody suitable for detecting surface CD27 by flow cytometry.
• Consider titrating the antibody in your flow cytometry protocol to determine the optimal concentration for your cell type and experimental conditions.
• Include appropriate compensation controls when using PerCP/Cyanine5.5 to ensure accurate multicolor flow cytometry data.
• Validate the antibody in your specific sample system, as staining patterns may vary with activation state or cell lineage.

CamelBio: Your One-Stop Sourcing Bridge

This anti-CD27 monoclonal antibody supports flow cytometric analysis of T-cell subsets and immune monitoring applications. CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For CD27-related immuno-oncology research, we offer targeted sourcing solutions to meet your development needs from concept to clinic.