PerCP/Cyanine5.5 Mouse anti-Human CD3 Monoclonal Antibody for FC - P07766

Core Product Specifications and Parameters

Biological Background: T-cell surface glycoprotein CD3 epsilon chain (CD3E) Function and Localization

• CD3E (CD3 epsilon) is an indispensable component of the TCR-CD3 complex on T lymphocytes, playing a central role in adaptive immunity by mediating T-cell activation upon antigen recognition. Related references: PMID:15294938 PMID:15546002 PMID:2470098
• Together with CD3D, CD3G, and CD247/CD3Z, CD3E transmits TCR engagement signals across the plasma membrane through its cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). Related references: PMID:2470098 PMID:40592325
• Upon TCR ligation, Src family kinases LCK and FYN phosphorylate the CD3E ITAMs, creating docking sites for ZAP70 kinase and initiating downstream activation cascades. Related references: PMID:2470098 PMID:40592325
• CD3E also contains endocytosis sequences that facilitate internalization and down-regulation of the TCR-CD3 complex from the cell surface. Related references: PMID:10384095 PMID:26507128
• Beyond its coreceptor function, CD3E acts as a receptor for ITPRIPL1, which inhibits T-cell activation by recruiting NCK1 and preventing CD3E-ZAP70 interaction, thereby blocking ERK-NFkB signaling and calcium influx. Related references: PMID:12110186 PMID:38614099
• CD3E is localized at the cell membrane as a single-pass type I membrane protein and undergoes disulfide bond formation and phosphorylation.

Experimental Guidance and Technical Tips

• The antibody was raised against recombinant human CD3 protein; consider verifying epitope specificity if required for your assay.
• As a fluorochrome-conjugated monoclonal antibody, it is suitable for direct flow cytometry. Optimize the titer in your experimental system.
• For multi-color panels, check compatibility of PerCP/Cyanine5.5 with other fluorophores and ensure proper compensation controls.
• Although validated for human samples, cross-reactivity with other species should be confirmed if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized support for rare targets. This PerCP/Cyanine5.5 anti-human CD3 monoclonal antibody adds to our flow cytometry portfolio, supporting T-cell research from concept to clinic. Contact us for seamless procurement and technical consultation.