PerCP/Cyanine5.5 Anti-Mouse CD69 Monoclonal Antibody for Flow Cytometry - P37217

Core Product Specifications and Parameters

Biological Background: CD69 Function and Localization

• CD69 (Early activation antigen) is a transmembrane C-type lectin glycoprotein rapidly upregulated on activated platelets, T lymphocytes, and NK cells, triggering distinct signaling cascades (PubMed:20696842, PubMed:38540778). Related references: PMID:20696842 PMID:38540778
• It negatively regulates Th17 differentiation by interacting with galectin-1/LGALS1 on dendritic cells and via JAK3/STAT5-mediated RORγt transcriptional control (PubMed:20696842). Related references: PMID:20696842
• CD69 promotes regulatory T cell conversion through S100A8/S100A9 and acts as an oxidized LDL receptor on CD4 T cells to induce PDCD1 expression, dampening inflammation.
• In eosinophils, CD69 ligation induces IL-10 secretion via ERK1/2 signaling, contributing to immune resolution (PubMed:38540778). Related references: PMID:38540778
• CD69 acts as a sphingosine-1-phosphate receptor 1 (S1PR1) agonist, promoting receptor internalization and degradation, thereby limiting lymphocyte and dendritic cell migration toward S1P gradients (PubMed:16525420). Related references: PMID:16525420
• Expressed on the surface of activated T cells, B cells, NK cells, neutrophils, platelets, and eosinophils; localized to the cell membrane with a single transmembrane domain.
• Post-translational modifications include glycosylation, phosphorylation, and disulfide bond formation, with a molecular weight of ~22.5 kDa.

Experimental Guidance and Technical Tips

• For flow cytometry, validate the antibody using mouse splenocytes or purified T cells stimulated with PMA/ionomycin or anti-CD3/CD28 for 4–6 hours.
• Include appropriate compensation controls when using PerCP/Cyanine5.5 in multicolor panels to address spectral overlap.
• Titrate the antibody (e.g., 0.1–1 µg per million cells) to determine the optimal staining concentration for your specific system.
• Consider surface staining with live/dead exclusion and fixation/permeabilization if combined with intracellular markers; verify PerCP/Cyanine5.5 stability under fixation conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop sourcing solution for IVD raw materials, supporting assay development from concept to clinic. Our portfolio includes validated antibody pairs, monoclonal antibodies, and ancillary reagents, with expertise in immunological targets such as CD69. Leverage our supply chain and technical services to accelerate your diagnostic or research applications.