PerCP/Cyanine5.5 anti-Mouse CD11c Monoclonal Antibody for FC - Q9QXH4

Core Product Specifications and Parameters

Biological Background: Integrin alpha-X (CD11c) Function and Localization

• Integrin alpha-X, also known as CD11 antigen-like family member C, leukocyte adhesion glycoprotein p150,95 alpha chain, or leukocyte adhesion receptor p150,95, is a single-pass type I membrane protein.
• As part of the integrin alpha-X/beta-2 complex, it serves as a receptor for fibrinogen, recognizing the G-P-R sequence, and plays a critical role in mediating cell-cell interactions during inflammatory responses, particularly in monocyte adhesion and chemotaxis.
• It also functions as a receptor for the erythrocyte-specific adhesion molecule ICAM4, mediating erythrophagocytosis.
• Subcellular localization is primarily at the cell membrane, consistent with its role in adhesion and signaling.
• The protein undergoes extensive post-translational modifications, including glycosylation and disulfide bond formation, which are essential for its structural integrity and function.
• It contains calcium and magnesium ion binding sites, indicative of its metal-dependent integrin activity.

Experimental Guidance and Technical Tips

• This antibody is directly conjugated to PerCP/Cyanine5.5, making it ready for flow cytometry without additional secondary staining steps.
• As a monoclonal antibody raised against a recombinant protein, it likely recognizes a linear epitope; however, performance in native versus denatured conditions should be validated in your specific experimental setup.
• For flow cytometry, titrate the antibody to determine the optimal concentration for the best signal-to-noise ratio in your cell system.
• Always include appropriate compensation controls (single-stained cells or beads) when using in multicolor panels, due to the spectral properties of the PerCP/Cyanine5.5 tandem dye.
• Note that the antibody is specific for mouse CD11c; cross-reactivity with other species has not been tested and is not expected unless specified.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, technical services, and consulting, covering every stage from concept to clinic. This anti-mouse CD11c antibody is a valuable tool for immunology research, particularly studies on monocyte biology, inflammation, and cell adhesion. Beyond single reagents, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and bespoke sourcing for rare targets, helping to streamline your development workflows and accelerate time-to-result.