PerCP Rabbit anti-Mouse CD8a Monoclonal Antibody for Flow Cytometry - P01731

Core Product Specifications and Parameters

Biological Background: CD8a Function and Localization

• T-cell surface glycoprotein CD8 alpha chain (CD8a, also known as Lyt-2) is an integral membrane glycoprotein essential for adaptive immunity.
• Serves as a coreceptor for MHC class I molecules: it simultaneously binds the T-cell receptor (TCR) and MHC class I on antigen-presenting cells, stabilizing the interaction.
• Recruits the Src kinase LCK to the TCR-CD3 complex, initiating intracellular signaling cascades that lead to lymphokine production, T-cell motility, adhesion, and cytotoxic T-lymphocyte (CTL) activation.
• In natural killer (NK) cells, CD8A homodimers promote survival and enhance conjugation with target cells, enabling serial lysis of multiple targets.
• Localizes to the cell membrane as a single-pass type I membrane protein.
• Undergoes post-translational modifications including N-linked glycosylation, disulfide bond formation, and palmitoylation, which regulate membrane localization and function.
• Alternative splicing generates multiple isoforms, though functional differences remain to be fully characterized.

Experimental Guidance and Technical Tips

• This PerCP-conjugated antibody is ready for direct flow cytometry; titrate to determine the optimal staining concentration for your mouse lymphocyte samples.
• The immunogen (amino acids 28–196) covers the extracellular domain, ensuring recognition of cell surface CD8a; validate with appropriate cell surface markers.
• Use mouse splenocytes, thymocytes, or peripheral blood lymphocytes as positive control samples, and include relevant isotype controls.
• As the target is surface-exposed, permeabilization is typically unnecessary; however, consider fixation conditions compatible with PerCP fluorophore stability.
• Cross-reactivity is reported only for mouse; verify species specificity if using other model organisms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement, offering a comprehensive portfolio from monoclonal antibodies to bulk ancillary reagents. This anti-mouse CD8a antibody supports T-cell immunology research, complementing our range of validated antibody pairs and custom raw-material sourcing for rare targets. Contact us to explore tailored supply solutions for your diagnostic or research needs.