PerCP Rabbit anti-Mouse CD44 Monoclonal Antibody for FC - P15379

Core Product Specifications and Parameters

Biological Background: CD44 Function and Localization

• CD44 is a cell-surface receptor that mediates cell-cell interactions, adhesion, and migration, allowing cells to sense and adapt to tissue microenvironment changes.
• Through its ectodomain, CD44 binds extracellular matrix components like hyaluronan and collagen, while its cytoplasmic domain assembles signaling complexes with kinases and RhoGTPases to regulate cytoskeletal reorganization and calcium mobilization.
  Related references: PMID:25065622 PMID:8343954
• It plays essential roles in T‑lymphocyte homing and recirculation, hematopoiesis, inflammation, and response to bacterial infection.
• Interaction with LGALS9 ligand triggers downstream LCK, ERK, and MAPK signaling, promoting NK cell activation.
  Related references: PMID:37006235
• CD44 localizes to the cell membrane, microvilli, and can also be secreted; it is expressed in the intestinal epithelium.
• Alternative splicing produces multiple protein isoforms, with observed molecular weights of 86, 81, 76, and 72 kDa; the protein is subject to glycosylation, phosphorylation, and disulfide bond formation.

Experimental Guidance and Technical Tips

• The PerCP conjugate enables direct detection by flow cytometry (FC) without secondary antibody steps; always include an appropriate isotype control.
• Titrate the antibody to determine optimal staining concentration for your cell population, as CD44 expression levels vary among different leukocyte subsets.
• Validate staining specificity using knockout or knockdown controls if possible, especially when analyzing rare or heterogeneous cell types.
• Since CD44 is widely expressed on hematopoietic cells, consider using multi‑color panels with lineage markers to gate on specific populations of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic and research laboratories with a comprehensive portfolio of IVD raw materials, including validated monoclonal antibodies like anti‑CD44 clones, recombinant proteins, and ancillary reagents. Our sourcing network simplifies procurement of bulk‑quantity PerCP‑conjugated antibodies, rare target antigens, and customized reagents for flow cytometry and other applications. Whether you require technical consulting or reliable supply chain solutions from concept to clinic, CamelBio serves as your single‑point connection.