PerCP Rabbit anti-Mouse CD209b/DC-SIGNR1 Monoclonal Antibody for FC - Q8CJ91

Core Product Specifications and Parameters

Biological Background: CD209b/DC-SIGNR1 Function and Localization

• CD209b, also known as DC-SIGNR1 or SIGNR1, is a mouse C-type lectin receptor (CLR) that likely functions as a pathogen-recognition receptor.
• It binds to high mannose N-linked oligosaccharides in a calcium-dependent manner, enabling interaction with microbial glycans and promoting endocytosis of pathogens for lysosomal degradation.
• The receptor is a type II transmembrane protein, anchored in the membrane via a signal-anchor sequence, and contains a carbohydrate recognition domain (CRD) responsible for ligand binding.
• It is expressed in skin, spleen, and lung, predominantly in subsets of dendritic cells, with localization observed in spleen extrafollicular paracortical areas and skin suprabasal layers.
• CD209b exhibits alternative splicing, producing multiple isoforms with molecular weights ranging from 16 kDa to 37 kDa, as indicated by UniProt and corroborated by product data.
• Key post-translational modifications include disulfide bond formation and glycosylation, essential for structural stability and lectin activity.
• As a DC-SIGN-related molecule, it plays a role in cell adhesion through interactions with ICAM-3, facilitating dendritic cell-mediated immune responses.

Experimental Guidance and Technical Tips

• The immunogen encompasses the extracellular ligand-binding domain, which may support detection of native conformations in flow cytometry.
• For FC, consider using appropriate isotype controls and titrating the antibody to optimize staining specificity.
• The presence of multiple isoforms may result in varied band sizes if used in Western blot; however, this product is validated for FC, so confirm application suitability.
• Since CD209b is expressed on dendritic cells, validate in relevant tissue or cell samples (e.g., spleen, skin, lung) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for diagnostic manufacturers and research labs, providing this PerCP-conjugated rabbit anti-mouse CD209b monoclonal antibody alongside a comprehensive portfolio of validated antibody pairs, recombinant proteins, and bulk ancillary reagents. For projects in dendritic cell biology and pathogen recognition, our one-stop sourcing includes rare target materials and technical support to accelerate your assay development from concept to clinic.