PerCP Rabbit anti-Human/Monkey CD45 Monoclonal Antibody for Flow Cytometry - P08575

Core Product Specifications and Parameters

Biological Background: CD45 (Receptor-type tyrosine-protein phosphatase C) Function and Localization

• Functions as a protein tyrosine phosphatase required for T-cell activation through the antigen receptor, as evidenced by genetic studies. It positively regulates T-cell coactivation upon binding to DPP4 and dephosphorylates downstream effectors including SKAP1, FYN, and LYN. Related references: PMID:35767951
• Acts as a receptor for human cytomegalovirus (HCMV) protein UL11, mediating UL11 binding to T-cells. This interaction dampens T-cell receptor signaling, reducing tyrosine phosphorylation and impairing T-cell proliferation.
• Primarily localized to the cell membrane, with enrichment in membrane rafts and at the immunological synapse, facilitating its role in signal transduction.
• Multiple isoforms arise from alternative splicing of PTPRC. Isoforms 1, 2, 3, 5, and 7 are detected in thymocytes, while isoforms 4, 6, and 8 are absent, indicating tightly regulated isoform-specific expression patterns.
• CD45 is a heavily glycosylated single-pass type I transmembrane protein with a predicted molecular weight of approximately 147 kDa, though detected isoforms range from 131 to 147 kDa. It contains two intracellular phosphatase domains and belongs to the protein-tyrosine phosphatase family.
• Classified under keywords including alternative splicing, glycoprotein, phosphoprotein, synapse, host-virus interaction, and SCID-related disease variant, reflecting its multifaceted roles.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to amino acids 26-577 of human CD45, covering a large extracellular region. This design favors detection of surface-expressed CD45 in live, unfixed cells, making it well-suited for flow cytometry applications.
• As a PerCP conjugate, consider spectral overlap with PE, PE-Cy5, and tandem dyes in multicolor panels. Use appropriate compensation controls and titrate the antibody for optimal signal-to-noise ratio.
• The antibody cross-reacts with cynomolgus CD45; validate staining patterns using relevant positive (e.g., human PBMCs, cynomolgus blood lymphocytes) and negative (e.g., CD45-negative cell lines) controls.
• For T-cell studies, note that isoform expression varies among T-cell subsets and differentiation stages. Pair with lineage-specific markers to accurately gate populations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with integrated access to IVD-grade antibodies and supporting raw materials. This anti-CD45 monoclonal antibody supports immunophenotyping and immune cell characterization workflows, essential for hematology and immuno-oncology applications. Beyond individual reagents, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assist in sourcing rare target raw materials to streamline your assay development from concept to clinic.