PerCP Rabbit anti-Human/Monkey CD4 Monoclonal Antibody for Flow Cytometry - P01730

Core Product Specifications and Parameters

Biological Background: CD4 Function and Localization

• T-cell surface glycoprotein CD4 (CD4) is a 51 kDa type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. It is also known as T4, Leu-3, and OKT4D.
• CD4 serves as a co-receptor for the T-cell receptor (TCR) by binding simultaneously to MHC class II molecules on antigen-presenting cells, thereby enhancing T-cell sensitivity and activation. Upon engagement, it recruits the Src kinase LCK to the TCR-CD3 complex, initiating intracellular signaling cascades that promote lymphokine production, motility, adhesion, and T-helper cell differentiation.
• CD4 is primarily expressed on the surface of T-helper cells, but can also be found on macrophages, dendritic cells, and natural killer (NK) cells, where it may participate in differentiation, activation, and migration in a TCR-independent manner.
• In the thymus, CD4 plays an essential role in the positive selection and maturation of T-helper cells, ensuring proper adaptive immune function.
• As a key host factor in viral pathogenesis, CD4 functions as the primary receptor for human immunodeficiency virus-1 (HIV-1), facilitating viral entry. It is downregulated by HIV-1 Vpu and also serves as a receptor for human herpesvirus 7 (HHV-7). Related references: PMID:12089508 PMID:16331979 PMID:2214026 PMID:17346169 PMID:7909607
• Post-translational modifications include N-linked glycosylation, disulfide bond formation, palmitoylation, and phosphorylation, which are critical for its stability, membrane localization, and interaction with signaling partners.

Experimental Guidance and Technical Tips

• For flow cytometry, it is recommended to titrate the antibody to determine the optimal dilution for your specific cell system.
• Consider including an appropriate isotype control to assess non-specific binding.
• This antibody may be used for surface staining of viable cells. Due to the PerCP conjugate, careful compensation controls are advised in multicolor flow cytometry experiments.
• Validate reactivity in the relevant species (human or cynomolgus) using positive controls such as peripheral blood mononuclear cells (PBMCs).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing platform for IVD raw materials, including monoclonal antibodies, recombinant proteins, and ancillary reagents. This anti-CD4 antibody supports research in immunophenotyping, HIV receptor studies, and T-cell biology, areas where CamelBio offers validated antibody pairs and bulk reagents. Leverage CamelBio's supply chain to access rare target antibodies and technical consulting services.