PerCP Mouse anti-Human CD3 Monoclonal Antibody for FC - P07766

Core Product Specifications and Parameters

Biological Background: CD3 Epsilon Chain Function and Localization

• The CD3 epsilon chain (CD3e) is a core component of the T-cell receptor (TCR)-CD3 complex on T-lymphocyte surfaces, essential for adaptive immune responses. Related references: PMID:15294938 PMID:15546002 PMID:2470098
• Upon TCR engagement by antigen-presenting cells, CD3e immunoreceptor tyrosine-based activation motifs (ITAMs) are phosphorylated by Src family kinases LCK and FYN, initiating downstream signaling. Related references: PMID:2470098 PMID:40592325
• CD3e ITAM phosphorylation creates docking sites for the protein kinase ZAP70, leading to its activation and transduction of signals crucial for T-cell activation, proliferation, and differentiation.
• CD3e plays an essential role in correct T-cell development, ensuring proper thymocyte maturation.
• The protein mediates internalization and cell surface down-regulation of the TCR-CD3 complex via endocytosis sequences in its cytosolic region. Related references: PMID:10384095 PMID:26507128
• Beyond TCR coreceptor function, CD3e also serves as a receptor for ITPRIPL1, which inhibits T-cell activation by promoting NCK1 interaction, thereby blocking ZAP70 binding and downstream ERK-NFkB signaling. Related references: PMID:38614099 PMID:12110186
• CD3e is localized to the cell membrane as a single-pass type I transmembrane protein, featuring an extracellular immunoglobulin-like domain and a cytoplasmic tail containing ITAMs.
• Key functional keywords associated with CD3e include adaptive immunity, cell membrane, disulfide bond, immunity, immunoglobulin domain, phosphoprotein, receptor, signal, transmembrane.

Experimental Guidance and Technical Tips

• The immunogen used is recombinant human CD3 protein; for flow cytometry, ensure cell surface staining protocols include appropriate blocking and washing steps to minimize nonspecific binding.
• Titrate the antibody to determine the optimal concentration for specific detection of CD3 on human T cells; consider using isotype controls to assess background staining.
• As a PerCP-conjugated antibody, pay careful attention to compensation settings in multicolor flow cytometry panels to avoid spectral overlap.
• Validate the antibody in relevant sample systems, such as peripheral blood mononuclear cells (PBMCs) or lymphoid tissues, to confirm expected staining patterns on T lymphocytes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For T-cell immunology research and diagnostic development targeting CD3, we facilitate access to high-quality raw materials, including rare target sourcing, to support your workflow from concept to clinic.