PerCP-conjugated Rabbit Anti-Mouse CD4 Monoclonal Antibody for FC - P06332

Core Product Specifications and Parameters

Biological Background: CD4 Function and Localization

• CD4, also known as L3T4 or Ly-4, is an integral membrane glycoprotein that functions as a T-cell co-receptor for MHC class II molecules.
• It interacts with the T-cell receptor (TCR) and MHC class II on antigen-presenting cells, recruiting the Src kinase LCK to initiate intracellular signaling pathways.
• This signaling leads to lymphokine production, T-cell motility, adhesion, and activation of T-helper cells.
• CD4 is highly expressed in T-helper cells and is also present in macrophages, dendritic cells, and NK cells.
• In the thymus, CD4 participates in T-helper cell development, and it triggers differentiation of monocytes into mature macrophages.
• CD4 is localized to the cell membrane and undergoes post-translational modifications such as glycosylation, disulfide bond formation, and palmitoylation.
• Keywords associated with CD4 include adaptive immunity, alternative splicing, immunoglobulin domain, and transmembrane helix.

Experimental Guidance and Technical Tips

• For flow cytometry applications, titrate the PerCP-conjugated antibody to determine the optimal staining concentration for your specific cell population.
• Use appropriate isotype control and compensation controls when performing multicolor flow cytometry.
• CD4 is expressed on T-helper cells, monocytes/macrophages, dendritic cells, and NK cells; validate in the relevant mouse tissue (e.g., spleen, lymph nodes, blood).
• Consider co-staining with anti-CD3 to identify T cells or with other lineage markers for immune cell profiling.
• Protect PerCP-conjugated antibodies from prolonged light exposure to prevent photobleaching.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides one-stop access to IVD raw materials, technical services, and consulting for diagnostic manufacturers and research labs. This PerCP-conjugated anti-mouse CD4 antibody supports immunology and immune cell profiling applications, complementing CamelBio's portfolio of validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and rare target sourcing capabilities.