PerCP anti-Mouse CD45 (Ptprc) Monoclonal Antibody for Flow Cytometry - P06800

Core Product Specifications and Parameters

Biological Background: CD45 Function and Localization

• CD45 (Receptor-type tyrosine-protein phosphatase C), encoded by the Ptprc gene, is a transmembrane glycoprotein and a member of the protein tyrosine phosphatase family. It is also known as Leukocyte common antigen (L-CA), Ly-5, and T200.
• The protein undergoes extensive alternative splicing, generating multiple isoforms with molecular weights ranging from 130 kDa to 144 kDa, as detected by this antibody.
• CD45 functions as a positive regulator of T-cell receptor (TCR) signaling by dephosphorylating the inhibitory tyrosine residues of Src family kinases such as LYN, thereby promoting T-cell activation.
• Upon T-cell activation, CD45 recruits and dephosphorylates downstream effectors including SKAP1 and FYN, modulating signal transduction cascades.
• CD45 interacts with DPP4, enhancing T-cell coactivation, and with CLEC10A on dendritic cells, which recognizes Tn antigen-carrying CD45 on effector T cells to regulate activation thresholds and limit autoreactivity.
• Subcellularly, CD45 is localized to the cell membrane, membrane rafts, and immunological synapses, positioning it at key sites for immune signal initiation.
• The protein is heavily glycosylated and phosphorylated, modifications essential for its structural integrity and enzymatic activity.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 26–566 of mouse CD45 (extracellular domain), suggesting epitope(s) are in the extracellular region accessible for live-cell flow cytometry.
• For flow cytometry, consider titrating the antibody to optimize staining index and reduce background. Include appropriate isotype controls and viability dyes.
• Since CD45 is expressed on all leukocytes, ensure proper gating strategies to identify cell populations of interest.
• Validate antibody performance in your specific sample type (e.g., spleen, lymph node, blood) as antigen density may vary.
• Avoid repeated freeze-thaw cycles; store as recommended.

CamelBio: Your One-Stop Sourcing Bridge

As a comprehensive IVD raw-material partner, CamelBio bridges diagnostic manufacturers and researchers with high-quality reagents like this anti-CD45 monoclonal antibody. Whether you need validated antibody pairs, bulk ancillary reagents, or customized sourcing for rare targets, our team supports your workflow from concept to clinic. Explore our portfolio to accelerate your immune cell phenotyping studies.