PerCP Anti-FOXP3 Monoclonal Antibody for Flow Cytometry (Intracellular) - Q9BZS1

Core Product Specifications and Parameters

Biological Background: FOXP3 Function and Localization

• FOXP3 is a forkhead box transcription factor essential for the development and suppressive function of regulatory T cells (Tregs). It maintains immune homeostasis by enabling Treg lineage stability and full suppressive activity. Related references: PMID:21458306 PMID:23947341 PMID:23169781
• It functions as both a transcriptional repressor and activator, depending on interaction partners, regulating genes like CTLA4, TNFRSF18, IL2, and IFNG in Tregs. Related references: PMID:17377532
• FOXP3 represses IL2 and IFNG expression via histone deacetylase recruitment (HDAC7) and by antagonizing transcription factors RELA and NFATC2. Related references: PMID:15790681 PMID:17360565
• It inhibits Th17 differentiation by antagonizing RORC and RORA, favoring Treg development and suppressing IL17. Related references: PMID:18368049 PMID:18354202
• Localizes to both nucleus and cytoplasm, with DNA-binding zinc-finger domains; post-translational modifications include acetylation, phosphorylation, and ubiquitination.
• Mutations in FOXP3 cause IPEX syndrome (immune dysregulation, polyendocrinopathy, enteropathy, X-linked), highlighting its critical role in immune tolerance.

Experimental Guidance and Technical Tips

• This PerCP-conjugated rabbit monoclonal targets the N-terminal region (aa 1-100) of human FOXP3. For intracellular flow cytometry, optimize fixation/permeabilization protocols to preserve epitope accessibility.
• Since PerCP exhibits moderate photostability, design panel compensation carefully and avoid prolonged laser exposure.
• Validate staining in known FOXP3-positive Treg populations (e.g., CD4+CD25+ cells) from human, mouse, or cynomolgus samples to confirm expected reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides end-to-end IVD raw material solutions, from validated antibody pairs to bulk conjugate reagents. This FOXP3 antibody supports immune monitoring and Treg research—ideal for diagnostic developers requiring consistent lot-to-lot performance. Inquire about customized labeling, large-volume supply, and ancillary assay components.