PerCP Anti-CD34 Monoclonal Antibody for Flow Cytometry - P28906

Core Product Specifications and Parameters

Biological Background: CD34 Function and Localization

• CD34 is a hematopoietic progenitor cell antigen that likely functions as an adhesion molecule.
• It plays a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells.
• CD34 could act as a scaffold for lineage-specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components.
• The protein presents carbohydrate ligands to selectins, facilitating cell-cell interactions.
• CD34 is selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
• Subcellularly, it is localized to the membrane as a single-pass transmembrane protein.
• Keywords associated with CD34 include alternative splicing, glycoprotein, phosphoprotein, signal peptide, and transmembrane.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 170–290 of human CD34, a region within the extracellular domain; this PerCP conjugate is suitable for direct flow cytometry detection without secondary antibodies.
• Surface staining of live cells is recommended, as the target is a transmembrane protein; consider using hematopoietic cell lines (e.g., KG-1, MOLM-13) or primary endothelial cells for validation.
• For consistent results, titrate the antibody using relevant positive and negative cell populations and include appropriate isotype controls.
• Note that CD34 expression can be modulated; verify staining patterns under your specific experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including this anti-CD34 antibody for hematopoietic stem cell research applications. Beyond individual reagents, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing—all supported by technical consulting to accelerate your concept-to-clinic journey.