PER2 Rabbit Monoclonal Antibody (Human) for WB, ELISA, ChIP - O15055

Core Product Specifications and Parameters

Biological Background: PER2 Function and Localization

• PER2 (Period circadian protein homolog 2), also known as circadian clock protein PERIOD 2, is a core component of the molecular circadian clock. It functions as a transcriptional repressor within the transcription/translation feedback loop (TTFL) that drives approximately 24-hour rhythms in gene expression.
• Together with PER1, CRY1, and CRY2, PER2 forms the negative limb of the TTFL. It interacts directly with CLOCK-BMAL1 heterodimers at E-box promoters to inhibit their own expression, while post‑translational modifications (e.g., phosphorylation, acetylation, ubiquitination) fine‑tune the period (tau) of circadian rhythms.
• PER2 contributes to clock‑controlled repression through multiple modes: it recruits SIN3‑HDAC chromatin‑remodeling complexes and H3K9 methyltransferases (SUV39H1/2) to clock gene promoters, and it also interacts with RNA‑binding proteins and helicases at 3′ termination sites to modulate transcription termination.
• The protein is implicated in metabolic and cardiovascular regulation. It acts as a coactivator of PPARA and a corepressor of NR1D1, rhythmically binding to promoters of genes such as BMAL1 and G6PC1, and it represses PPARG‑mediated adipogenesis. PER2 also regulates nitric oxide and prostaglandin production in aortas and controls glutamate uptake via VGLUT1 expression.
• Bi‑allelic loss‑of‑function mutations in PER2 cause familial advanced sleep phase syndrome (FASPS), underscoring its essential role in entrainment of the circadian clock to environmental light‑dark cycles.
• PER2 localizes primarily to the nucleus but is also found in the cytoplasm, perinuclear region, and nucleolus, reflecting dynamic shuttling and compartment‑specific functions.
• The protein is widely expressed, with high levels in skeletal muscle and pancreas, and lower levels in lung. Isoform 2 is specifically detected in keratinocytes.
• Key structural features include two PAS domains and a C‑terminal cytoplasmic localization domain that contribute to protein‑protein interactions and subcellular trafficking.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 1–100 of human PER2 (O15055), an N‑terminal region that is well‑conserved and likely to generate antibodies recognizing both full‑length and truncated isoforms.
• For western blotting (WB), expect a band at approximately 137 kDa; use nuclear or whole‑cell lysates from human tissues or cell lines known to express PER2 (e.g., skeletal muscle, pancreas). Endogenous PER2 levels oscillate over the circadian cycle—consider collecting samples at defined zeitgeber times.
• For ELISA applications, coupling the antibody with a detection reagent targeting a non‑overlapping epitope can improve sensitivity and specificity; validate dynamic range using recombinant PER2 protein or controlled cell lysates.
• In chromatin immunoprecipitation (ChIP), the antibody may enrich E‑box‑containing promoter regions of clock‑controlled genes (e.g., PER1, CRY1). Include appropriate negative control regions and confirm enrichment by qPCR under synchronized clock conditions.
• Given the role of post‑translational modifications in PER2 function, consider using phosphatase inhibitors or denaturing conditions if studying phosphorylation‑dependent interactions or localization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of high‑quality IVD raw materials, technical services, and consulting—from concept to clinic. This PER2 monoclonal antibody supports circadian rhythm, sleep disorder, and metabolic research, complementing our extensive range of validated antibodies, antigen pairs, and ancillary reagents. Leverage our expertise to accelerate your assay development with reliable, batch‑to‑batch consistent materials and customized sourcing solutions for rare or emerging targets.