PE/Cyanine7 Rat anti-Mouse MHC Class II (I-A/I-E) Monoclonal Antibody for FC - P18468 / P06342 / Q3U060 / P14435

Core Product Specifications and Parameters

Biological Background: MHC Class II (I-A/I-E) Function and Localization

• The target protein includes the H-2 class II histocompatibility antigen, I-A beta chain (encoded by H2-Eb1), a core component of the MHC class II complex.
• It is a transmembrane glycoprotein localized to the membrane, featuring a signal peptide, transmembrane helix, and disulfide bond(s) as indicated by the UniProt keywords.
• Functionally, it plays a critical role in adaptive immunity by presenting peptide antigens to CD4+ T cells, consistent with its classification as an MHC II molecule involved in immune responses.
• The protein is subject to ubiquitin-like (Ubl) conjugation, a post‑translational modification that may regulate its trafficking or stability.
• It belongs to the MHC class II protein family and is a member of the reference proteome, indicating a well‑characterized sequence and structural annotation.
• The I-A beta chain has an expected molecular weight of approximately 30 kDa; additional bands around 32 kDa and 26 kDa likely correspond to related MHC class II chains (e.g., I-E beta, I-A alpha) that share high homology and are co‑targeted by this antibody.

Experimental Guidance and Technical Tips

• For flow cytometry, use a cell surface staining protocol with live, unfixed cells to preserve membrane protein integrity.
• Due to the presence of multiple MHC class II isoforms and chain associations, validate antibody specificity in the mouse strain and cell type relevant to your experiment.
• The recombinant protein immunogen covers broad sequence regions; consider epitope mapping if discrimination between I-A and I-E chains is critical for your study.
• When designing multicolor panels, include proper compensation controls for the PE/Cyanine7 conjugate to avoid spectral overlap issues.
• Confirm cross‑reactivity in your sample system, as the antibody is raised against mouse antigen and is reported to react with mouse but may not detect orthologs from other species.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes streamlined access to IVD raw materials, including validated monoclonal antibodies like this anti‑MHC Class II clone. Beyond antibodies, we can supply matched antibody pairs, optimized ancillary reagents, and bulk quantities to support your immunological assay development and validation. Partner with us to efficiently source rare‑target raw materials and accelerate your project from concept to clinic.