PE/Cyanine7 Rat anti-Mouse CD3 Monoclonal Antibody for Flow Cytometry - P04235 / P22646

Core Product Specifications and Parameters

Biological Background: CD3 Function and Localization

• CD3 is a core component of the T-cell receptor (TCR) complex on T lymphocytes, essential for adaptive immune responses.
• The TCR-CD3 complex comprises CD3 delta, epsilon, gamma, and zeta chains; this antibody targets both CD3 delta (P04235) and CD3 epsilon (P22646).
• Upon TCR engagement by antigen-presenting cells, the immunoreceptor tyrosine-based activation motifs (ITAMs) of CD3 chains are phosphorylated by Src-family kinases LCK and FYN, initiating downstream signaling cascades.
• CD3 delta (CD3D) is critical for thymocyte differentiation and correct intracellular assembly and surface expression of the TCR-CD3 complex; in its absence, thymocytes fail to differentiate properly. Related references: PMID:10935641
• CD3D interacts directly with CD4 and CD8 co-receptors, linking TCR recognition to T-cell activation and positive selection of CD4+ or CD8+ T-cells.
• CD3 delta is a transmembrane glycoprotein located at the cell membrane, featuring key post-translational modifications such as glycosylation and phosphorylation.
• Keywords associated with CD3D include adaptive immunity, receptor, phosphoprotein, and glycoprotein, underscoring its central role in immune signaling.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide derived from mouse CD3; consider titrating the antibody to determine the optimal concentration for flow cytometry.
• PE/Cyanine7 is a tandem conjugate; protect from prolonged light exposure and use appropriate compensation controls to avoid spillover issues.
• Include proper controls such as isotype control and fluorescence-minus-one (FMO) controls to validate staining specificity.
• Verify cross-reactivity and epitope recognition in your specific mouse tissue or cell type, as CD3 expression may vary among T-cell subsets.

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CamelBio offers a comprehensive one-stop platform for IVD raw materials, supporting diagnostic developers and researchers with fully characterized reagents like this PE/Cyanine7 anti-mouse CD3 antibody. Whether you need optimized antibody pairs, bulk ancillary reagents, or customized monoclonal development for immune-oncology panels, our supply chain ensures consistent quality from concept to clinic. Let us accelerate your T-cell assay development with reliable raw-material sourcing.