PE/Cyanine7 Rabbit anti-Mouse/Rat CD31/PECAM1 Monoclonal Antibody for Flow Cytometry - Q08481

Core Product Specifications and Parameters

Biological Background: CD31/PECAM1 Function and Localization

• PECAM1 (CD31) is an 81 kDa cell adhesion molecule and member of the immunoglobulin superfamily, with alternative splicing generating multiple isoforms.
• Essential for leukocyte transendothelial migration (TEM) during inflammation; Tyr-679 phosphorylation is critical for trafficking from the lateral border recycling compartment (LBRC) to sites of leukocyte passage.
• Mediates homophilic endothelial cell-cell adhesion at junctions and heterophilic interaction with CD177 to facilitate neutrophil TEM.
• Prevents macrophage-mediated phagocytosis of viable leukocytes through detachment signaling, while promoting phagocytosis of apoptotic cells by tethering them to phagocytes.
• Modulates bradykinin receptor BDKRB2 activation and hyperosmotic stress-induced ERK1/2 signaling in endothelial cells.
• Associated with atherosclerosis susceptibility (PubMed:19048083). Related references: PMID:19048083
• Subcellularly located at cell membrane, membrane rafts, and cell junctions.
• Expressed predominantly in lung and platelets (at protein level).

Experimental Guidance and Technical Tips

• The PE/Cyanine7 conjugate is optimized for flow cytometry; titrate carefully and include appropriate isotype and fluorescence minus one (FMO) controls.
• Immunogen comprises extracellular amino acids 18–220, enabling recognition of surface-exposed CD31 on live cells without permeabilization.
• Validate staining in CD31-expressing samples such as lung tissue homogenates, purified platelets, or cultured endothelial cells.
• Co-stain with lineage markers (e.g., CD45 for leukocytes, VE-cadherin for endothelial cells) to resolve mixed populations.
• Minimize exposure to light and avoid fixation if it quenches the tandem dye; include viability dyes to exclude dead cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers one-stop access to IVD raw materials, technical services, and consulting, supporting diagnostic manufacturers from concept to clinic. Our PE/Cyanine7 anti-CD31/PECAM1 monoclonal antibody, targeting a key adhesion molecule in vascular biology and inflammation, enables reliable flow cytometric analysis for research and assay development. Complement your projects with our full range of validated antibodies, bulk ancillary reagents, and rare target raw-material sourcing solutions.