PE/Cyanine7 Rabbit anti-Mouse Ki67 Monoclonal Antibody for Flow Cytometry (Intracellular) - E9PVX6

Core Product Specifications and Parameters

Biological Background: Ki67 Function and Localization

• Ki67 is a well-established proliferation marker encoded by the Mki67 gene; alternative names include Antigen KI-67, Ki-67, and D630048A14Rik.
• Ki67 associates with mitotic chromosomes, forming brush-like structures that act as a surfactant to disperse chromosomes and prevent their collapse into a single chromatin mass (PubMed:27362226). Related references: PMID:27362226
• This protein exhibits a dual role: during early mitosis it serves as a chromosome repellent, while in late anaphase it transitions to a chromosome attractant, facilitating chromosome clustering through liquid-liquid phase separation.
• Ki67 does not contribute to the internal architecture of mitotic chromosomes, suggesting its primary role is in surface-mediated organization (PubMed:26949251). Related references: PMID:26949251
• Subcellular localization includes the nucleus, nucleolus, and chromosome surfaces, consistent with its cell-cycle-dependent dynamics.
• Ki67 expression is tightly linked to cell proliferation, being predominantly detected in cycling cells and widely used as a marker for growth fraction.
• Post-translational modifications such as phosphorylation and acetylation regulate its function, and it contains ATP-binding and DNA-binding domains, with a preference for supercoiled and AT-rich DNA.
• The protein undergoes alternative splicing, generating multiple isoforms that may contribute to its diverse cellular roles.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1816-2163 of mouse Ki67; consider verifying specificity in knockout or knockdown models.
• For intracellular flow cytometry (FC), optimize fixation and permeabilization protocols to ensure adequate antibody access to nuclear Ki67.
• Co-staining with DNA dyes or other cell-cycle markers may enhance analysis of proliferating cell populations.
• Validate the antibody’s performance in the relevant mouse tissue or cell line, using appropriate isotype controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio acts as a one-stop sourcing bridge for IVD raw materials, connecting diagnostic developers with high-quality reagents like this anti-Ki67 monoclonal antibody. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, with expert support for rare-target procurement. Whether you need proliferation markers for oncology assay development or custom antibody sourcing, CamelBio streamlines your path from concept to clinic.