PE/Cyanine7 Rabbit anti-Mouse CD95/FAS Monoclonal Antibody for FC - P25446

Core Product Specifications and Parameters

Biological Background: CD95/FAS Function and Localization

• Tumor necrosis factor receptor superfamily member 6 (FAS, also known as CD95, Apo-1, APT1, TNFR6, Tnfrsf6) is encoded by the Fas gene in mouse.
• Functions as a receptor for TNFSF6/FASLG; upon ligand binding, recruits FADD and activates caspase CASP8 via the death-inducing signaling complex (DISC), initiating the caspase cascade that leads to apoptosis.
• FAS-mediated apoptosis may contribute to peripheral tolerance and the antigen-stimulated suicide of mature T-cells.
• Localizes to the cell membrane and membrane rafts.
• Detected in various tissues including thymus, liver, lung, heart, and adult ovary.
• Post-translational modifications include disulfide bonds, glycosylation, and palmitoylation (glycoprotein, lipoprotein, palmitate, phosphoprotein).
• Belongs to the tumor necrosis factor receptor superfamily; contains extracellular cysteine-rich repeats characteristic of the family.

Experimental Guidance and Technical Tips

• Immunogen design: The antibody was generated using a recombinant fusion protein corresponding to amino acids 22-169 of mouse CD95/FAS, covering the extracellular domain, which is appropriate for recognizing native receptor on the cell surface.
• Validated for flow cytometry (FC); ensure appropriate isotype controls and titration in the relevant mouse sample system.
• Because the antibody is conjugated to PE/Cyanine7, avoid exposure to light and use appropriate compensation controls if multiplexing.
• CD95 expression can be induced or modulated; consider validating in stimulated versus unstimulated conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing platform for IVD raw materials, technical services, and consulting, supporting diagnostic manufacturers from concept to clinic. For apoptosis and immune regulation research involving CD95/FAS, we can supply validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets. Contact us to discuss your specific procurement needs.