PE/Cyanine7 Rabbit anti-Mouse CD8a Monoclonal Antibody for FC - P01731

Core Product Specifications and Parameters

Biological Background: CD8a Function and Localization

• CD8a (T-cell surface glycoprotein CD8 alpha chain), also known as Lyt-2, is an integral membrane glycoprotein essential for adaptive immunity.
• It functions as a coreceptor for MHC class I molecules on antigen-presenting cells, enabling CD8+ T cells to recognize peptide-MHC I complexes and initiate cytotoxic responses against infected or tumor cells.
• Upon TCR engagement, CD8a recruits the Src kinase LCK to the TCR-CD3 complex, triggering phosphorylation cascades that lead to lymphokine production, adhesion, and CTL activation.
• In NK cells, CD8a homodimers support survival and serial killing of target cells by facilitating conjugation and lysis.
• CD8a signaling promotes differentiation of activated lymphocytes into memory CD8+ T cells, sustaining long-term immunity.
• The protein localizes exclusively to the cell membrane as a single-pass type I membrane protein, with an extracellular immunoglobulin domain, a transmembrane helix, and a short cytoplasmic tail.
• Post-translational modifications include disulfide bond formation, N-linked glycosylation, and palmitoylation, which may influence membrane organization and signaling.
• Alternative splicing produces multiple isoforms, though the full-length isoform is predominant in T cells.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 28–196) of mouse CD8a, avoiding the transmembrane and cytoplasmic regions; this design is expected to yield antibodies recognizing the native cell-surface conformation.
• This PE/Cyanine7 conjugate is validated for flow cytometry; ensure appropriate titration (e.g., 0.25–1 µg per 10^6 cells) and include isotype controls when analyzing mouse lymphocytes.
• Because CD8a is co-expressed with TCR, consider co-staining with anti-CD3 to distinguish CD8+ T cells from NK cells, which may also express CD8a homodimers.
• Validate specificity in CD8a-deficient mouse strains or by blocking with unconjugated anti-CD8a to confirm binding.
• Avoid prolonged light exposure to preserve fluorophore stability; use within 24 hours of reconstitution if diluted.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents. This anti-mouse CD8a monoclonal antibody supports immune profiling and T-cell monitoring, and CamelBio can source additional CD8a clones, custom conjugates, or matched detection reagents to streamline your assay development. As a B2B partner, we simplify procurement of rare-target antibodies and bulk raw materials, helping you scale from concept to clinic.