PE/Cyanine7 Rabbit anti-Mouse CD62L Monoclonal Antibody for Flow Cytometry - P18337

Core Product Specifications and Parameters

Biological Background: CD62L (L-selectin) Function and Localization

• CD62L (L-selectin), encoded by the Sell gene, is a calcium-dependent cell adhesion molecule belonging to the selectin family. It is also known as LAM-1, LECAM1, and Ly-22, with a molecular weight of approximately 42 kDa.
• It mediates lymphocyte adhesion to high endothelial venules (HEV) in peripheral lymph nodes by binding to glycoprotein ligands on endothelial cells, facilitating lymphocyte homing (PubMed:1693096). Related references: PMID:1693096
• L-selectin promotes the initial tethering and rolling of leukocytes along activated endothelium, a critical early step in the leukocyte adhesion cascade (By similarity).
• Structurally, it contains an N-terminal C-type lectin domain, an EGF-like domain, and short consensus repeat (Sushi) domains, followed by a transmembrane helix and a short cytoplasmic tail.
• The protein is localized on the cell membrane, specifically on the surface of leukocytes, including lymphocytes, neutrophils, and monocytes.
• It is predominantly expressed in lymphoid tissues, consistent with its role in lymphocyte trafficking.
• Post-translational modifications include glycosylation and disulfide bond formation, as indicated by the glycoprotein and disulfide bond keywords.
• As a key molecule in immune surveillance, CD62L is often used as a marker for naive and central memory T cells in flow cytometry applications.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 39-332 of mouse CD62L, encompassing the extracellular region. This design should generate antibodies that recognize the native membrane-bound form of L-selectin.
• This PE/Cyanine7-conjugated monoclonal antibody is suitable for direct flow cytometry without the need for a secondary detection step. Consider titrating the antibody to determine the optimal staining concentration for your specific cell preparation.
• Since CD62L is shed from the cell surface upon leukocyte activation, ensure appropriate sample handling to preserve its expression. Fixation and permeabilization protocols may affect epitope recognition; validate in the relevant sample system.
• Use appropriate isotype controls and viability dyes to distinguish specific staining. CD62L expression is typically analyzed on peripheral blood leukocytes, splenocytes, or lymph node cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and assay developers with a streamlined channel to source this PE/Cyanine7 Rabbit anti-Mouse CD62L monoclonal antibody along with a comprehensive range of IVD raw materials. In addition to pre-conjugated antibodies, we supply matched antibody pairs, bulk ancillary reagents, and custom antibody development support—enabling you to accelerate immune-monitoring and biomarker discovery projects from concept to clinic.