PE/Cyanine7 Rabbit anti-Mouse CD44 Monoclonal Antibody for FC - P15379

Core Product Specifications and Parameters

Biological Background: CD44 Function and Localization

• CD44 is a multifunctional cell-surface receptor and hyaluronan-binding protein that mediates cell-cell and cell-matrix adhesion, critical for lymphocyte homing, hematopoiesis, and inflammatory responses. Related references: PMID:25065622 PMID:8343954
• Its ectodomain engages extracellular matrix components (hyaluronan, collagen, growth factors), while the cytoplasmic domain assembles signaling complexes with kinases (e.g., PKN2) and RhoGTPases (RAC1, RHOA) to coordinate cytoskeletal remodeling and cell migration.
• Upon LGALS9 binding, CD44 triggers downstream LCK, ERK, and MAPK activation to promote NK cell cytotoxicity. Related references: PMID:37006235
• The protein localizes to the plasma membrane and microvillus projections; a secreted ectodomain form is generated by proteolytic shedding.
• Extensive post-translational modifications—including alternative splicing, N- and O-glycosylation, phosphorylation, and sulfation—yield numerous isoforms with distinct functional properties.
• In mice, tissue-specific expression is prominent in the intestinal epithelium.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal hyaluronan-binding ectodomain (amino acids 23–176), suggesting reactivity with both standard and variant CD44 isoforms; confirm isoform specificity in your model if required.
• For flow cytometry, titrate the antibody individually to determine the optimal concentration for your cell type and instrument settings to achieve clear population separation.
• Include appropriate compensation controls when using PE/Cyanine7 tandem dye to avoid spectral overlap artifacts.
• Validate antibody performance under your experimental conditions (e.g., cell stimulation, fixation/permeabilization) as these may affect epitope accessibility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines procurement for diagnostic developers and research labs seeking high-quality IVD raw materials. This PE/Cyanine7 anti-mouse CD44 antibody supports flow cytometry applications in lymphocyte biology, inflammation, and cancer research. As your integrated supply partner, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to accelerate your projects from concept to clinic.