PE/Cyanine7 Rabbit anti-Mouse CD4 Monoclonal Antibody for Flow Cytometry - P06332

Core Product Specifications and Parameters

Biological Background: CD4 Function and Localization

• T-cell surface glycoprotein CD4 (also designated L3T4 and Ly-4) is a type I transmembrane protein belonging to the immunoglobulin superfamily.
• Functions as a co-receptor for MHC class II molecules on antigen-presenting cells, enhancing T-cell receptor (TCR) signaling.
• Upon ligand engagement, CD4 recruits the Src kinase LCK to the TCR-CD3 complex, initiating phosphorylation cascades that promote T-cell activation, lymphokine production, and adhesion.
• Plays a critical role in thymic T-helper cell development and triggers monocyte-to-macrophage differentiation in a TCR-independent manner.
• Primarily expressed on the surface of T-helper cells, but also present on macrophages, dendritic cells, and NK cells.
• Subcellular location is the plasma membrane, with a single-pass transmembrane domain and a cytoplasmic tail involved in signal transduction.
• Subject to multiple post-translational modifications including N-linked glycosylation, palmitoylation, disulfide bond formation, and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 27-394) of mouse CD4, supporting recognition of surface-expressed antigen.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific mouse cell population.
• Validate staining with known T-helper cell controls (e.g., CD4+ T-cell lines or primary mouse splenocytes).
• Consider co-staining with anti-CD3 or anti-CD8 to characterize T-cell subsets.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your integrated sourcing partner for IVD raw materials, offering a comprehensive selection of validated antibodies for immunology and diagnostics. Our PE/Cyanine7 anti-mouse CD4 monoclonal antibody facilitates reliable flow cytometry analysis in murine models. Through a one-stop service model, CamelBio supports your project with antibody pairs, bulk reagents, and custom raw-material procurement from development to commercial scale.