PE/Cyanine7 Rabbit anti-Mouse CD38 Monoclonal Antibody for Flow Cytometry - P56528

Core Product Specifications and Parameters

Biological Background: Mouse CD38 Function and Localization

• ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 (also known as ADPRC 1, I-19, NIM-R5 antigen) is a multifunctional transmembrane glycoprotein encoded by the Cd38 gene.
• Enzymatically, CD38 synthesizes cyclic ADP-ribose (cADPR) and nicotinate-adenine dinucleotide phosphate (NAADP+), both potent second messengers for calcium mobilization; it also exhibits cADPR hydrolase activity.
  Related references: PMID:11829748
• CD38 acts as a receptor for CD31 (PECAM-1) on endothelial cells, triggering lymphocyte activation, proliferation, and transendothelial migration; in lamina propria T cells, CD31 engagement activates LCK and LAT, leading to cytokine release.
• The protein is a single-pass type II transmembrane protein (signal-anchor) located on the cell membrane, with a transmembrane helix.
• Post-translational modifications include N-linked glycosylation and disulfide bond formation.
• CD38 is involved in dendritic cell maturation, regulating CCL21-driven migration, survival, and Th1-polarizing ability.
• Its molecular weight is approximately 34 kDa (calculated from sequence).

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (aa 45-304) of mouse CD38, suggesting the antibody may recognize an epitope accessible on live cells; consider this when designing flow cytometry experiments.
• PE/Cyanine7 emits in the far-red channel; plan appropriate compensation when combining with other fluorophores in multicolor panels.
• For optimal staining, titrate the antibody and include relevant isotype controls in your flow cytometry protocol.
• Reported cross-reactivity is with mouse; reactivity with other species should be experimentally validated if needed.
• To maintain conjugate stability and antibody activity, avoid repeated freeze-thaw cycles and store as recommended.

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