PE/Cyanine7 Rabbit anti-Mouse CD34 Monoclonal Antibody for FC - Q64314

Core Product Specifications and Parameters

Biological Background: Hematopoietic progenitor cell antigen CD34 Function and Localization

• CD34 is a transmembrane glycoprotein that functions as a potential adhesion molecule, facilitating the attachment of hematopoietic stem cells to the bone marrow extracellular matrix or stromal cells.
• Acts as a scaffold for lineage-specific glycans, enabling stem cell adhesion via lectins on stromal cells and presenting carbohydrate ligands to selectins.
• Localizes to the membrane, consistent with its role in cell-cell and cell-matrix interactions.
• In mouse, CD34 is highly expressed in hematopoietic progenitor cell lines, brain, and testis, with moderate expression in thymus, spleen, and bone marrow, but absent in adult liver. Related references: PMID:1709048
• Expression is also detected in the kidney, specifically in the thin limb of Henle's loop. Related references: PMID:31605441
• Classified as a phosphoprotein and glycoprotein, with alternative splicing generating variant isoforms.
• Contains a signal peptide and a single-pass transmembrane helix, characteristic of cell surface receptors.

Experimental Guidance and Technical Tips

• As a PE/Cyanine7 tandem conjugate, protect the antibody from light and minimize freeze-thaw cycles to preserve fluorescence intensity.
• The immunogen spans the extracellular region (aa 35-287), supporting detection of surface CD34 by flow cytometry; consider using an isotype control and titrate for optimal staining.
• CD34 is a classical marker for hematopoietic stem cells; validate performance in relevant samples such as mouse bone marrow, splenocytes, or hematopoietic cell lines.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials and technical services. This anti-mouse CD34 antibody serves hematopoietic stem cell research, hematology, and cell adhesion studies. From validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and rare target sourcing, CamelBio streamlines your procurement from concept to clinic.