PE/Cyanine7 Rabbit anti-Mouse CD25 Monoclonal Antibody for FC - P01590

Core Product Specifications and Parameters

Biological Background: CD25 Function and Localization

• CD25 is the alpha subunit of the interleukin-2 receptor, also referred to as p55 or IL-2RA.
• It functions as a high-affinity receptor for IL-2, playing a critical role in immune homeostasis.
• CD25 is a key regulator of immune tolerance by controlling the activity of regulatory T cells (TREGs), which suppress autoreactive T-cell activation and expansion.
• The protein is a type I transmembrane glycoprotein located on the cell membrane, with an extracellular region containing multiple sushi (CCP) domains.
• Post-translational modifications include glycosylation and disulfide bond formation, essential for its structure and function.
• CD25 expression is upregulated upon T-cell activation and serves as a classical marker for Treg identification in mouse models.

Experimental Guidance and Technical Tips

• The PE/Cyanine7 direct conjugate enables one-step staining, minimizing handling time and reducing the need for secondary reagents in flow cytometry.
• Optimize the antibody working concentration by titration in the relevant mouse cell system to achieve the best staining index.
• Include appropriate isotype controls and fluorescence-minus-one (FMO) controls to ensure specific signal detection.
• Because the immunogen covers the extracellular domain (aa 22-236), this antibody is well-suited for surface staining of live cells; if intracellular detection is required, validate fixation/permeabilization protocols with this clone.
• For Treg identification, co-staining with FoxP3 or other markers may be performed after surface staining, but check compatibility of fixation buffers with the PE/Cyanine7 fluorophore.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw-material sourcing, offering a wide range of validated antibodies, including conjugated monoclonals like this anti-CD25 product. Whether you need optimized antibody pairs, bulk reagents, or assistance sourcing rare targets for immunology research, CamelBio bridges the gap from concept to clinic with technical service and supply chain support.