PE/Cyanine7 Rabbit anti-Mouse CD23 Monoclonal Antibody for Flow Cytometry - P20693

Core Product Specifications and Parameters

Biological Background: CD23 Function and Localization

• CD23, also known as Fc-epsilon-RII and lymphocyte IgE receptor, is a low-affinity receptor for immunoglobulin E (IgE) and complement receptor 2 (CD21/CR2).
• On B cells, CD23 regulates IgE production and drives B cell differentiation, and it mediates IgE-dependent antigen uptake and presentation to T cells.
• On macrophages, IgE binding and antigen cross-linking through CD23 activates the L-arginine–nitric oxide pathway, leading to intracellular killing of parasites.
• The receptor is primarily localized to the cell membrane via a signal-anchor transmembrane domain, but a secreted form is also produced through alternative splicing.
• CD23 is a type II transmembrane glycoprotein with C-type lectin domains, and it undergoes post-translational modifications including palmitoylation, proteoglycan modification, and disulfide bond formation.
• Metal-binding calcium ions are essential for its structural integrity and ligand-binding function.
• The mouse Fcer2 gene encodes CD23 and belongs to the C-type lectin receptor family; it generates multiple isoforms by alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen covers almost the entire extracellular domain (aa 50–331), likely preserving native conformational epitopes, which may be beneficial for detection of cell surface CD23 by flow cytometry.
• PE/Cyanine7 is a tandem conjugate suitable for multicolor flow cytometry panels; ensure proper compensation controls are included when co-staining with other fluorochromes.
• This antibody has been validated for mouse samples; cross-reactivity with other species has not been tested and should be evaluated if applied to non-murine systems.
• Consider titrating the antibody to determine the optimal concentration for your specific experimental setup to minimize background and maximize sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials, covering the entire development journey from concept to clinic. This PE/Cyanine7 anti-mouse CD23 monoclonal antibody exemplifies our capability to supply validated immunological reagents for B cell and IgE studies. We offer a comprehensive portfolio, including optimized antibody pairs, bulk ancillary reagents, and bespoke sourcing for rare target raw materials—backed by technical and regulatory consulting to accelerate your diagnostic development.