PE/Cyanine7 Rabbit anti-Mouse CD206/MMR Monoclonal Antibody for Flow Cytometry - Q61830

Core Product Specifications and Parameters

Biological Background: CD206/MMR (Macrophage Mannose Receptor 1) Function and Localization

• MRC1 encodes the Macrophage Mannose Receptor 1 (CD206), a type I transmembrane glycoprotein that serves as a pattern recognition receptor.
• Functions as an endocytic receptor, binding both sulfated and non-sulfated polysaccharide chains on glycoproteins to mediate their internalization in macrophages.
• Acts as a phagocytic receptor for bacteria, fungi, and other pathogens, contributing to innate immune defense.
• Localizes predominantly to the cell membrane and cycles to the endosome membrane upon ligand binding and internalization.
• Contains multiple extracellular domains, including cysteine-rich, fibronectin type II, and C-type lectin domains, which facilitate calcium-dependent carbohydrate recognition.
• Detected specifically in macrophages, consistent with its role in pathogen clearance.
• Undergoes post-translational glycosylation and disulfide bond formation, typical of cell surface receptors.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell population, and include proper compensation controls when using PE/Cyanine7 conjugate.
• Protect the antibody from prolonged light exposure to minimize fluorochrome photobleaching.
• For intracellular staining, validate fixation and permeabilization protocols to preserve epitope accessibility, as the immunogen corresponds to an N-terminal extracellular region.
• Consider using macrophages or macrophage-like cell lines as a positive control and include isotype controls to assess non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies this PE/Cyanine7-conjugated anti-mouse CD206 monoclonal antibody as part of a comprehensive portfolio for immunological research and diagnostic development. Our one-stop access covers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to support your workflow from concept to clinic.