PE/Cyanine7 Rabbit anti-Mouse CD19 Monoclonal Antibody for Flow Cytometry - P25918

Core Product Specifications and Parameters

Biological Background: CD19 Function and Localization

• B-lymphocyte antigen CD19 (Differentiation antigen CD19) is a transmembrane glycoprotein coreceptor of the B-cell antigen receptor complex, reducing the activation threshold for B-cell responses.
• The coreceptor activates phosphatidylinositol 3-kinase signaling and intracellular calcium mobilization, essential for B-cell functions. Related references: PMID:12387743, PMID:20101619, PMID:9382888
• CD19 is dispensable for early bone marrow B-cell development but is essential for B-1 cell differentiation. Related references: PMID:12387743, PMID:7542548, PMID:7543183, PMID:9317126
• It is required for antigen-induced B-cell proliferation and differentiation. Related references: PMID:12387743, PMID:7542548, PMID:9317126
• CD19 is critical for maintaining normal serum immunoglobulin levels and generating high-affinity antibodies. Related references: PMID:12387743, PMID:7542548, PMID:7543183
• The protein localizes to the plasma membrane and is enriched in membrane rafts, facilitating signaling complex assembly.
• Post-translational modifications include disulfide bond formation, N-linked glycosylation, and phosphorylation, which modulate stability and function.
• CD19 is predominantly expressed on B cells in spleen, bone marrow, thymus, lymph nodes, and peripheral blood. Related references: PMID:12387743, PMID:20101619, PMID:7542548, PMID:7543183

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal extracellular domain (amino acids 19-287), suggesting the antibody likely recognizes an accessible epitope on live cells, supporting flow cytometry without permeabilization.
• As a PE/Cyanine7 conjugate, this antibody enables direct detection; verify that your instrument configuration accommodates PE/Cy7 excitation and emission filters.
• For optimal flow cytometry performance, titrate the antibody to your specific cell type and include appropriate isotype and fluorescence-minus-one controls to assess specificity and signal-to-noise ratio.
• Validate the antibody in a relevant mouse B-cell line (e.g., A20, WEHI-231) or primary splenocytes, and consider Fc receptor blocking to reduce non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we provide a seamless supply chain of IVD raw materials for diagnostic developers, including validated monoclonal antibodies like anti-CD19 conjugates. Our portfolio supports B-cell immunology research and IVD kit manufacturing with optimized antibodies, bulk ancillary reagents, and expert sourcing for rare targets. Partner with us to accelerate your assay development from concept to clinic.