PE/Cyanine7 Rabbit anti-Mouse CD11b Monoclonal Antibody for Flow Cytometry - P05555

Core Product Specifications and Parameters

Biological Background: Integrin alpha-M Function and Localization

• Integrin alpha-M (ITGAM, CD11b) is a leukocyte-specific adhesion receptor that pairs with the beta-2 integrin chain (CD18) to form the Mac-1 (CR3) complex.
• It functions as a receptor for the iC3b fragment of complement component C3, facilitating opsonized phagocytosis and clearance of pathogens.
• Also binds fibrinogen, factor X, and ICAM1, mediating leukocyte adhesion, migration, and transendothelial trafficking.
• In microglia, ITGAM/ITGB2 together with TYROBP/DAP12 controls production of superoxide ions, promoting developmental neuronal apoptosis. Related references: PMID:18685038
• Subcellular localization: Cell membrane and membrane rafts, consistent with its role in cell adhesion and signaling.
• Predominantly expressed in monocytes and granulocytes, with additional expression in peritoneal mast cells and brain microglia.
• Post-translational modifications include disulfide bonds, glycosylation, and alternative splicing; contains calcium-binding and metal-binding domains critical for ligand recognition.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 143-337 within the extracellular domain of mouse CD11b. This region may be accessible for antibody binding in flow cytometry without permeabilization; however, testing under native and fixed/permeabilized conditions is recommended to confirm reactivity.
• PE/Cyanine7 tandem dye conjugates are highly sensitive but susceptible to photobleaching and may require careful handling and minimal light exposure. Use appropriate laser excitation (e.g., blue or yellow-green) and emission filters.
• Validate antibody dilution in the intended sample type (e.g., mouse splenocytes, peripheral blood mononuclear cells) by titration to determine optimal staining index.
• Cross-reactivity is reported as mouse. No data are provided for other species; testing should be performed if extended reactivity is needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering seamless access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Whether you require PE/Cyanine7 Rabbit anti-Mouse CD11b mAb for flow cytometry-based diagnostic assays or complementary reagents like anti-CD18 antibodies for co-immunoprecipitation, our technical and supply chain teams bridge the gap from concept to clinical application. Reach out to explore batch reservations, custom conjugation services, and flexible procurement options.