PE/Cyanine7 Rabbit anti-Mouse CD105/Endoglin Monoclonal Antibody for Flow Cytometry - Q63961

Core Product Specifications and Parameters

Biological Background: Endoglin Function and Localization

• Endoglin is a transmembrane glycoprotein primarily expressed on vascular endothelial cells, also known as cell surface MJ7/18 antigen.
• It plays an essential role in angiogenesis and is required for normal extraembryonic angiogenesis and embryonic heart development. Related references: PMID:10625534
• Endoglin regulates the migration of vascular endothelial cells, contributing to vascular remodeling. Related references: PMID:17540773
• It may regulate endothelial cell shape changes in response to blood flow, driving establishment of normal vascular morphology during angiogenesis. Related references: PMID:28530658
• Acts as a TGF-beta coreceptor, involved in TGF-beta/BMP signaling cascades leading to SMAD transcription factor activation. Related references: PMID:23300529
• Required for GDF2/BMP9 signaling through SMAD1 and modulates TGFB1 signaling through SMAD3.
• Subcellularly localized to the cell membrane; detected on endothelial cells of adult pulmonary artery, heart capillaries, and lung alveolar capillaries; also found in stromal cells of various organs. Related references: PMID:8194490, PMID:10625534
• Post-translational modifications include glycosylation and phosphorylation; classified as a disulfide-linked glycoprotein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 27-581) of mouse CD105, which may facilitate recognition of the native cell surface protein in flow cytometry.
• For flow cytometry, consider using appropriate isotype controls and titrate the antibody to determine optimal staining concentration for your cell type.
• Since CD105 is expressed on endothelial cells and some stromal cells, ensure proper gating strategies and include endothelial markers for identification.
• The antibody is validated for mouse reactivity; cross-reactivity with other species has not been tested. Validate in your experimental system if using other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with a streamlined sourcing solution for IVD raw materials. This anti-CD105/Endoglin antibody supports vascular biology and oncology applications, and CamelBio can supply validated antibody pairs, bulk reagents, and rare target materials to accelerate your development. Contact us for customized procurement and technical consultation.