PE/Cyanine7 Rabbit anti-Human/Monkey PD-1/CD279 Monoclonal Antibody for FC - Q15116

Core Product Specifications and Parameters

Biological Background: PD-1 Function and Localization

• PD-1 (Programmed cell death protein 1), also known as CD279, is an inhibitory receptor encoded by the PDCD1 gene that plays a critical role in induction and maintenance of immune tolerance. Related references: PMID:21276005, PMID:31754127, PMID:32184441
• It delivers inhibitory signals upon binding to its ligands CD274/PDCD1L1 and CD273/PDCD1LG2, downregulating T-cell responses. Related references: PMID:21276005, PMID:26602187
• Following T-cell receptor (TCR) engagement, PD-1 associates with TCR-CD3 in the immunological synapse and directly inhibits T-cell activation by recruiting PTPN11/SHP-2. Related references: PMID:32184441
• The SHP-2 phosphatase dephosphorylates key TCR proximal signaling molecules, including ZAP70, PRKCQ/PKCθ, and CD247/CD3ζ, thereby suppressing T-cell activation. Related references: PMID:32184441
• The PD-1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity; blockade of this pathway reverses exhausted T-cell phenotypes and restores anti-tumor responses, forming the basis of cancer immunotherapy. Related references: PMID:28951311, PMID:22658127, PMID:25034862, PMID:25399552
• PD-1 is a single-pass transmembrane glycoprotein localized to the cell membrane and contains an immunoglobulin-like domain, disulfide bonds, and intracellular signaling motifs subject to phosphorylation and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen used spans the extracellular domain (amino acids 25-168) of human PD-1, suggesting the antibody targets the native cell-surface conformation.
• When using this PE/Cyanine7-conjugated antibody for flow cytometry, ensure proper compensation settings and include appropriate isotype controls to account for non-specific binding.
• Cross-reactivity with cynomolgus monkey (Macaca fascicularis) PD-1 has been observed, potentially enabling translational studies in non-human primate models.
• It is advisable to titrate the antibody and validate its performance in the relevant sample system, as optimal conditions may vary depending on cell type and experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes a streamlined one-stop channel for IVD raw materials, covering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. For PD-1/immune checkpoint research and immunotherapy development, CamelBio can supply this conjugated anti-PD-1 antibody alongside complementary reagents to support your discovery and translational workflows. Our integrated supply model helps you move from concept to clinic with reliable material access and expert consultation.