PE/Cyanine7 Rabbit anti-Human/Monkey IgD Monoclonal Antibody for FC - P01880

Core Product Specifications and Parameters

Biological Background: Immunoglobulin heavy constant delta Function and Localization

• IgD is the constant region of immunoglobulin delta heavy chains, present as membrane-bound (mIgD) and secreted (sIgD) forms, functioning as a major B-cell antigen receptor. Related references: PMID:20176268, PMID:22158414
• Membrane-bound IgD, coexpressed with IgM on naive B cells, triggers clonal expansion and differentiation by phosphorylating CD79A/CD79B via Src kinases upon antigen binding. Related references: PMID:20176268, PMID:22158414
• Antigen-binding diversity arises from V(D)J recombination and subsequent somatic hypermutations, enabling affinity maturation for specific antigens. Related references: PMID:17576170, PMID:20176268
• Secreted IgD stimulates monocytes to release pro-inflammatory cytokines such as TNF, IL1B, IL6, IL10, LIF, and IL1RN, acting as a potent immune mediator. Related references: PMID:10702483, PMID:11282392, PMID:8774350
• IgD localizes to the cell membrane (transmembrane form) and is secreted into the serum, with membrane anchorage mediated by a transmembrane helix.
• The protein undergoes disulfide bonding and glycosylation, characteristic of immunoglobulin domains, and is identified through proteomic analysis.
• Alternative names include Ig delta chain C region, NIG-65, and WAH, reflecting allelic variants.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 99–384 of the human IgD constant region, ensuring target specificity; cross-reactivity with cynomolgus monkey extends its potential use in non-human primate models.
• For flow cytometry applications, validate the antibody in the intended sample system (e.g., human peripheral blood mononuclear cells) and employ proper isotype controls to confirm specific staining.
• Co-staining with an anti-IgM antibody can help identify naive B cells, where IgD and IgM are coexpressed; optimize fluorophore combinations to minimize spectral overlap.
• Since the antibody is PE/Cyanine7-conjugated, protect from light and monitor for potential tandem dye degradation during prolonged experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with consolidated access to validated IVD raw materials, including monoclonal and polyclonal antibodies, optimized antibody pairs, and bulk ancillary reagents. For B-cell immunology targets like IgD, we support flow cytometry and immunoassay development with rare-target sourcing and technical consultation, helping you accelerate from concept to clinic. Contact us to explore how our integrated supply chain can meet your immunoglobulin-based reagent needs.