PE/Cyanine7 Rabbit anti-Human/Monkey CD45RA mAb for FC - P08575-8

Core Product Specifications and Parameters

Biological Background: CD45RA Function and Localization

• CD45RA is isoform 6 of the receptor-type tyrosine-protein phosphatase C (PTPRC).
• It is also referred to as Leukocyte common antigen (L-CA) and T200.
• The PTPRC gene (commonly known as CD45) encodes this protein.
• The calculated molecular weight of this isoform is 138 kDa.

Experimental Guidance and Technical Tips

• For flow cytometry, consider titrating the antibody to determine the optimal concentration for your specific cell type.
• Validate staining with appropriate isotype controls to account for non-specific binding.
• Ensure compatibility of PE/Cyanine7 with your instrument’s laser and filter configuration (typically excited by a blue or yellow-green laser and detected in the 780/60 or equivalent channel).
• For dual-targeting, avoid spectral overlap with other fluorophores; check compensation settings.
• The antibody targets an extracellular epitope (amino acids 26-482), so surface staining of live cells is expected.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with integrated access to IVD raw materials, from validated antibody pairs and bulk ancillary reagents to customized monoclonal antibodies like this anti-CD45RA clone. Whether you need flow cytometry reagents, immunology research tools, or support scaling up your assay development, our team bridges every stage from concept to clinic.