PE/Cyanine7 Rabbit anti-Human/Monkey CD4 Monoclonal Antibody for FC - P01730

Core Product Specifications and Parameters

Biological Background: CD4 Function and Localization

• CD4 is an integral membrane glycoprotein essential for adaptive immune responses, acting as a coreceptor for MHC class II molecules on antigen-presenting cells to facilitate T-cell receptor (TCR) signaling.
• Upon interaction with MHC class II, CD4 recruits the Src kinase LCK to the TCR-CD3 complex, initiating intracellular signaling cascades that promote T-helper cell activation, lymphokine production, motility, and adhesion.
• In macrophages and NK cells, CD4 plays a TCR/LCK-independent role in differentiation, activation, cytokine expression, and cell migration.
• CD4 is the primary receptor for HIV-1, mediating viral entry (PubMed:12089508, PubMed:16331979, PubMed:2214026), and is down-regulated by HIV-1 Vpu (PubMed:17346169). It also serves as a receptor for Human Herpesvirus 7 (PubMed:7909607).
  Related references:
  PMID:12089508
  PMID:16331979
  PMID:2214026
  PMID:17346169
  PMID:7909607
• CD4 is predominantly localized at the cell membrane and is highly expressed on T-helper cells, with lower expression on macrophages, dendritic cells, and NK cells.
• The CD4 protein contains immunoglobulin-like domains, disulfide bonds, and is subject to glycosylation, palmitoylation, and phosphorylation, contributing to its structural and functional diversity.

Experimental Guidance and Technical Tips

• This PE/Cyanine7-conjugated monoclonal antibody is specifically validated for flow cytometry (FC) applications. For optimal staining, titrate the antibody to determine the appropriate dilution for your cell type and experimental conditions.
• CD4 is a cell surface marker; therefore, live-cell staining protocols with appropriate Fc receptor blocking are recommended to minimize nonspecific binding.
• Cross-reactivity has been demonstrated with cynomolgus monkey CD4, making it suitable for non-human primate immunophenotyping studies.
• If using this antibody in other applications (e.g., IHC, WB), verify specificity and sensitivity in the relevant sample system, as performance may vary across platforms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutions streamlined access to validated IVD raw materials, including monoclonal antibodies, antibody pairs, and ancillary reagents for immune cell profiling. As a comprehensive sourcing partner, we supply high-quality flow-cytometry-grade antibodies and can assist in sourcing rare-target raw materials to accelerate your T-cell and infectious disease research programs.