PE/Cyanine7 Rabbit anti-Human/Monkey CD3 Monoclonal Antibody for FC - P07766

Core Product Specifications and Parameters

Biological Background: CD3E Function and Localization

• CD3E is a core component of the TCR-CD3 complex expressed on T lymphocytes, essential for adaptive immune responses. Related references: PMID:15294938, PMID:15546002
• Upon TCR engagement, CD3E ITAMs are phosphorylated by Src kinases (LCK/FYN), recruiting ZAP70 and triggering downstream signaling cascades. Related references: PMID:2470098, PMID:40592325
• Critical for proper T-cell development and thymocyte selection (By similarity).
• Cytosolic endocytosis motifs mediate TCR-CD3 complex internalization and surface down-regulation. Related references: PMID:10384095, PMID:26507128
• Acts as an alternative receptor for ITPRIPL1; ligand binding inhibits T-cell activation via NCK1-mediated blockade of CD3E-ZAP70 interaction and ERK-NFkB signaling. Related references: PMID:12110186, PMID:38614099
• Single-pass type I membrane protein; extracellular region contains an immunoglobulin-like domain, cytoplasmic tail carries ITAMs.
• Classified under keywords: Adaptive immunity, cell membrane, disulfide bond, immunoglobulin domain, phosphoprotein, transmembrane helix.
• Calculated molecular mass is ~23.1 kDa; observed at 23 kDa.

Experimental Guidance and Technical Tips

• The antibody targets the extracellular domain of CD3E (aa 1–126), making it suitable for live-cell surface staining.
• Use appropriate isotype controls and viability dyes to discriminate live/dead cells during flow cytometry.
• Perform antibody titration to determine the optimal staining concentration for your sample.
• Protect the PE/Cyanine7 conjugate from prolonged light exposure to maintain fluorophore stability.
• Validate performance in relevant sample systems such as human peripheral blood mononuclear cells (PBMCs) or cynomolgus monkey lymphocytes.

CamelBio: Your One-Stop Sourcing Bridge

This CD3E monoclonal antibody exemplifies CamelBio’s commitment to providing high-quality IVD raw materials. As a one-stop sourcing platform, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can assist in obtaining rare-target raw materials for diagnostic development. Empower your T-cell immunophenotyping and immune research with reliable, ready-to-use reagents from CamelBio.