PE/Cyanine7 Rabbit anti-Human/Monkey CD25 Monoclonal Antibody for Flow Cytometry - P01589

Core Product Specifications and Parameters

Biological Background: CD25 (IL2RA) Function and Localization

• CD25 (Interleukin-2 receptor subunit alpha, also known as p55 or TAC antigen) is the alpha chain of the high-affinity IL-2 receptor, critical for immune homeostasis.
• It plays a key role in regulatory T cell (TREG) function by controlling IL-2 signaling, thereby maintaining self-tolerance and preventing autoimmunity.
• CD25 is a type I transmembrane glycoprotein located on the cell surface, characterized by extracellular sushi domains (short consensus repeats) involved in ligand binding.
• The protein contains intramolecular disulfide bonds and is heavily glycosylated, contributing to its structural stability and receptor activity.
• It is predominantly expressed on activated T cells, regulatory T cells, and some other immune subsets, serving as a well-established marker for T cell activation.
• Genetic variants in IL2RA are associated with immune disorders such as type 1 diabetes (IDDM10) and immunodeficiency 41 (IMD41), underscoring its role in immune regulation.
• UniProt keywords include Immunity, Receptor, Glycoprotein, Disulfide bond, Signal peptide, Transmembrane helix, and Sushi repeats.

Experimental Guidance and Technical Tips

• This PE/Cyanine7-conjugated antibody enables direct flow cytometric detection of CD25 without the need for secondary staining steps.
• As the immunogen corresponds to the extracellular domain (amino acids 22-213), the antibody is expected to recognize membrane-bound CD25 on live cells.
• Titrate the antibody to determine the optimal staining concentration for your specific cell type and assay conditions.
• Use appropriate isotype controls and viability dyes to exclude nonspecific binding and dead cells.
• When analyzing regulatory T cells, consider co-staining with CD4 and FoxP3 (intracellular) and ensure proper compensation for multicolor panels.
• CD25 expression can vary with activation status; validate on known positive (e.g., activated PBMCs) and negative (e.g., resting T cells) populations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, providing this PE/Cyanine7 anti-CD25 antibody alongside a comprehensive portfolio of immunology and immune-oncology reagents. Whether your project requires validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, or assistance with rare target raw-material procurement, CamelBio connects you with reliable supply solutions from concept to clinic. Let our expertise in CD25 and regulatory T cell biology streamline your diagnostic or research development.