PE/Cyanine7 Rabbit anti-Human/Monkey CD20 Monoclonal Antibody for FC - P11836

Core Product Specifications and Parameters

Biological Background: CD20 Function and Localization

• CD20, also known as B-lymphocyte surface antigen B1 or MS4A1, is a member of the membrane-spanning 4-domains subfamily A.
• It functions as a store-operated calcium channel component, regulating calcium influx upon B-cell receptor activation, essential for B-cell development, differentiation, and activation. Related references: PMID:12920111, PMID:3925015, PMID:7684739, PMID:18474602
• CD20 is expressed specifically on the surface of B-lymphocytes, making it a key marker for B-cell lineage and a target for immunotherapies.
• The protein localizes to the cell membrane.
• It undergoes post-translational modifications such as phosphorylation and palmitoylation, contributing to its function and membrane association.
• Alternative splicing produces multiple isoforms, with calculated molecular weights of approximately 33 kDa and 14 kDa, consistent with the observed protein weights.
• CD20 plays a role in B-cell activation and is a pharmaceutical target for monoclonal antibody therapies against B-cell malignancies and autoimmune disorders.

Experimental Guidance and Technical Tips

• Use this PE/Cyanine7-conjugated antibody for flow cytometric analysis of CD20 expression on human or cynomolgus monkey B-cells; validate in your specific sample system.
• The immunogen (aa 100-200 of human CD20) targets a region that may be exposed extracellularly; consider using surface staining protocols.
• For multiparameter flow cytometry, employ appropriate compensation controls and include isotype controls to ensure specificity.
• The protein has reported molecular weights of ~33 kDa and ~14 kDa due to alternative splicing; if performing immunoblotting, verify the detected band(s) in your system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw-material sourcing partner, supplying validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents for diagnostic development. This anti-CD20 antibody supports B-cell research and therapeutic monitoring, and we can assist with customized antibody conjugation, stability testing, and scalable production. Reach out for rare target sourcing or full workflow support.