PE/Cyanine7 Rabbit Anti-Human TIM-3/HAVCR2 Monoclonal Antibody for Flow Cytometry - Q8TDQ0

Core Product Specifications and Parameters

Biological Background: TIM-3/HAVCR2 Function and Localization

• TIM-3/HAVCR2 (CD366) is a cell surface receptor that fine-tunes innate and adaptive immunity, generally exerting inhibitory functions but able to co-stimulate depending on cellular context and ligand engagement.
  Related references: PMID:24825777
• It suppresses Th1-mediated auto- and alloimmune responses, promotes immunological tolerance, and attenuates CD8+ TCR signaling by blocking NF-κB/NFAT and reducing IL-2 secretion, likely via LCK association and LGALS9-dependent PTPRC recruitment; it also regulates macrophage activation.
  Related references: PMID:14556005 PMID:24337741 PMID:26492563 PMID:11823861
• On Treg cells, TIM-3 inhibits Th17 responses, while on NK cells it exhibits a dual role: enhancing IFN-γ production upon LGALS9 engagement and yet suppressing natural cytotoxicity.
  Related references: PMID:24838857 PMID:22323453 PMID:22383801
• It serves as a receptor for galectin-9 (LGALS9), which can trigger T-cell apoptosis and immunosuppression, though this interaction remains debated; TIM-3 also binds phosphatidylserine to mediate phagocytosis of apoptotic cells by dendritic cells.
  Related references: PMID:16286920 PMID:24337741 PMID:23555261
• Subcellularly, TIM-3 localizes to the cell membrane and cell junctions. Its expression spans Th1 lymphocytes, Tregs, dendritic cells, NK cells, and epithelial tissues, with marked upregulation in chronic viral infections such as HCV and HIV.
• Post-translationally, TIM-3 is a glycoprotein with disulfide bonds, palmitoylation, phosphorylation, and ubiquitination, reflecting regulated turnover and signaling interactions.

Experimental Guidance and Technical Tips

• The immunogen (aa 22‑200) covers the entire extracellular domain, making this antibody well‑suited for detecting surface TIM‑3 in live‑cell flow cytometry; consider using the recommended PE/Cyanine7 conjugate for bright signal and minimal spectral overlap with FITC and PE channels.
• Since TIM‑3 expression is highly dynamic and upregulated upon T‑cell activation, stimulate your cells (e.g., with anti‑CD3/CD28) or include known activating conditions to enhance detection sensitivity.
• When designing multicolor panels, account for PE/Cyanine7 photobleaching and non‑specific binding; titrate the antibody and include proper isotype and FMO controls to ensure accurate gating.
• The antibody is validated on human samples; cross‑reactivity with other species is not specified, so validate in your relevant sample system if working with non‑human cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a specialized one‑stop platform for IVD raw‑material procurement, supporting diagnostics developers from early R&D through clinical application. For immune checkpoint research—particularly studies targeting TIM-3/HAVCR2—we supply validated monoclonal antibodies, matched antibody pairs, bulk ancillary reagents, and can source custom rare‑target raw materials. Partner with CamelBio to streamline your supply chain and accelerate your immuno‑oncology or infectious disease assay development.