PE/Cyanine7 Rabbit anti-Human LILRB4/CD85k/ILT3 mAb - Q8NHJ6

Core Product Specifications and Parameters

Biological Background: LILRB4 Function and Localization

• LILRB4 is an inhibitory immune receptor that down-regulates immune responses and promotes immune tolerance, a key checkpoint in immune homeostasis. Related references: PMID:11875462
• It serves as a receptor for fibronectin (FN1), apolipoprotein E (APOE), and ALCAM/CD166, linking extracellular matrix and lipid metabolism to immune regulation. Related references: PMID:34089617 PMID:30333625 PMID:29263213
• The receptor inhibits monocyte activation by attenuating phosphorylation of signaling kinases LCK, SYK, LAT, and ERK, thereby suppressing TNF production. Related references: PMID:19833736 PMID:9151699
• LILRB4 suppresses T cell proliferation, induces anergy, and promotes the differentiation of CD8+ T suppressor cells, contributing to immune evasion in tumors. Related references: PMID:16493035 PMID:19833736 PMID:29263213
• The protein is expressed on the surface of monocytes, macrophages, dendritic cells, natural killer cells, and B cells, with prominent expression in the lung.
• Structurally, LILRB4 contains immunoglobulin-like domains and exists in multiple isoforms due to alternative splicing, reflecting its multifaceted regulatory roles.

Experimental Guidance and Technical Tips

• The immunogen comprises the extracellular domain (aa 22–259) of human LILRB4, targeting the ligand-binding region; confirm surface staining in flow cytometry protocols.
• For flow cytometry, titrate the antibody on relevant human immune cell lines or primary PBMCs to determine optimal concentration.
• Since LILRB4 is expressed on multiple leukocyte subsets, including monocytes and dendritic cells, include appropriate isotype and FMO controls to gate specific populations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, supporting diagnostic developers from concept to clinic. This antibody targets the immune checkpoint receptor LILRB4, a molecule of high interest in cancer immunology and autoimmune disease research. CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets to streamline your assay development.