PE/Cyanine7 Rabbit anti-Human IFN-gamma Monoclonal Antibody for Intracellular Flow Cytometry - P01579

Core Product Specifications and Parameters

Biological Background: Interferon gamma Function and Localization

• Interferon gamma (IFN-γ) is a type II interferon predominantly secreted by activated T lymphocytes and natural killer (NK) cells, acting as a key pleiotropic cytokine in immune regulation.
• It exerts crucial antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation.
  Related references:
  PMID:16914093
  PMID:8666937
• Upon binding to its receptor IFNGR1, IFN-γ triggers the JAK-STAT signaling cascade, leading to STAT1 activation, nuclear translocation, and transcription of IFN-γ-regulated genes, including transcription factors such as IRF1.
  Related references:
  PMID:8349687
  PMID:16914093
• IFN-γ enhances class I MHC antigen presentation by inducing the replacement of constitutive proteasome subunits with immunoproteasome subunits, and by upregulating the PA28 activator, thereby increasing the quantity, quality, and repertoire of peptides for MHC class I loading.
  Related references:
  PMID:8666937
  PMID:8163024
  PMID:11112687
• It also up-regulates MHC class II complexes on the cell surface by promoting the expression of key molecules such as cathepsins B, H, and L, further optimizing antigen presentation.
  Related references:
  PMID:7729559
• Participates in the regulation of hematopoietic stem cell development, quiescence, and differentiation under homeostatic conditions. (By similarity)
• IFN-γ is a secreted glycoprotein with a calculated molecular mass of approximately 19.3 kDa; it is characterized by a signal peptide, glycosylation, and a cleavage site on pair of basic residues (UniProt Keywords).

Experimental Guidance and Technical Tips

• For intracellular flow cytometry, establish appropriate cell fixation and permeabilization conditions to allow detection of IFN-γ in activated T cells or NK cells; common stimulants include PMA/ionomycin in the presence of a protein transport inhibitor.
• The immunogen (aa 24–166) corresponds to the mature secreted form of human IFN-γ; thus, the antibody is expected to recognize intracellularly accumulated cytokine, making it suitable for functional cytokine assays.
• Include proper compensation controls when using PE/Cyanine7 conjugate in multicolor panels, and consider using a dump channel or viability dye to exclude dead cells.
• Validate staining specificity in relevant positive and negative control samples, such as stimulated versus unstimulated human peripheral blood mononuclear cells, to confirm detection of endogenous IFN-γ.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material supplier, CamelBio provides diagnostic manufacturers and laboratories with streamlined access to high-performance antibodies and ancillary reagents for immunology and cytokine research. This PE/Cyanine7 anti-IFN-γ monoclonal antibody complements our portfolio of validated antibody pairs, optimized clones for ELISA and flow cytometry, and bulk ancillary reagents—enabling you to efficiently source the components you need from concept to clinic. Should you require custom raw-material sourcing or technical consultation, our team is ready to bridge your project requirements.