PE/Cyanine7 Rabbit anti-Human FcεR1α Monoclonal Antibody for FC - P12319

Core Product Specifications and Parameters

Biological Background: FcεR1α Function and Localization

• Protein identity: High affinity immunoglobulin epsilon receptor subunit alpha (FcεRIα), also known as Fc-epsilon RI-alpha or IgE Fc receptor subunit alpha, encoded by the FCER1A (FCE1A) gene.
• Core function: Serves as the high-affinity receptor for immunoglobulin E (IgE). Upon IgE binding and subsequent allergen cross-linking, it triggers signaling cascades leading to myeloid cell activation, degranulation, and release of vasoactive amines, lipid mediators, and cytokines.
• Immune response role: Mediates immediate hypersensitivity reactions to allergens and acts as a host defense mechanism against helminth parasites, pathogenic bacteria, and venom toxicity.
• Subcellular localization: Cell membrane; single-pass type I membrane protein.
• Tissue specificity: Expressed in eosinophils, mast cells, basophils, and other myeloid cells.
• Structural features: Glycoprotein with immunoglobulin-like domains, disulfide bonds, and a transmembrane helix. Belongs to the immunoglobulin superfamily.
• Disease relevance: Dysregulation of FcεRIα signaling can lead to severe allergic and anaphylactic reactions.
• Molecular weight: Approximately 30 kDa (glycosylated form) as observed by SDS-PAGE; the predicted unmodified mass is ~29.6 kDa.

Experimental Guidance and Technical Tips

• This antibody is directly conjugated to PE/Cyanine7 for flow cytometry (FC) applications, minimizing the need for secondary staining.
• The immunogen corresponds to the extracellular domain (amino acids 26–205) of human FcεR1α, ensuring recognition of the native cell surface receptor.
• Titrate the antibody to determine optimal staining concentration for your specific cell type, and include appropriate isotype controls.
• Validate specificity using FcεR1α-expressing cells such as eosinophils or basophils, or transfected cell lines.
• For multicolor FC panels, assess spectral overlap with other fluorochromes and design appropriate compensation controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive one-stop sourcing of IVD raw materials for diagnostic manufacturers, research laboratories, and institutes—covering every stage from concept to clinic. This anti-FcεR1α antibody supports allergy and inflammation research, backed by our reliable supply chains for monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material procurement. Contact us for validated antibody pairs, optimized conjugation services, and tailored sourcing solutions to accelerate your assay development.