PE/Cyanine7 Rabbit anti-Human CR2/CD21 Monoclonal Antibody for Flow Cytometry - P20023

Core Product Specifications and Parameters

Biological Background: CR2/CD21 Function and Localization

• CR2, also known as CD21 or complement C3d receptor, is a type I transmembrane glycoprotein that serves as a receptor for complement component C3d, facilitating antigen recognition and uptake by B-cells (PubMed:1849076, PubMed:21527715, PubMed:7753047). Related references: PMID:1849076 PMID:21527715 PMID:7753047
• It forms a B-cell coreceptor complex with CD19, FCRL5, and CD81, which is critical for B-cell activation and signaling (PubMed:1383329, PubMed:30107486). Related references: PMID:1383329 PMID:30107486
• In addition to CD19-dependent signaling, CR2 can activate SRC tyrosine kinase independently, leading to phosphorylation of nucleolin and downstream AKT and GSK3 kinase activities (PubMed:12938232). Related references: PMID:12938232
• CR2 also functions as a ligand for CD23 (FcεRII), a low-affinity IgE receptor, thereby participating in the regulation of IgE production (PubMed:1386409). Related references: PMID:1386409
• It acts as a receptor for the Epstein-Barr virus (EBV) on B-cells, mediating viral entry.
• Localized predominantly at the cell membrane.
• Expressed on mature B-lymphocytes, T-lymphocytes, pharyngeal epithelial cells, astrocytes, and follicular dendritic cells of the spleen.
• The protein features multiple sushi (CCP) domains, disulfide bonds, and undergoes alternative splicing, resulting in isoforms with calculated molecular weights of approximately 110–119 kDa.

Experimental Guidance and Technical Tips

• The immunogen used spans the entire extracellular domain (amino acids 21–971), ensuring recognition of native CR2 on intact cells, which is well-suited for flow cytometry applications.
• For FC assays, consider using this PE/Cyanine7-conjugated antibody at empirically determined dilutions, titrating on known CR2-positive B-cell lines (e.g., Raji) or primary human B-cells.
• Since CR2 is heavily glycosylated and has multiple isoforms, confirm specificity through knockout controls or competitive binding with recombinant C3d.

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