PE/Cyanine7 Rabbit anti-Human CD90/Thy1 Monoclonal Antibody for Flow Cytometry - P04216

Core Product Specifications and Parameters

Biological Background: CD90/Thy-1 Function and Localization

• CD90/Thy-1 is a GPI-anchored glycoprotein belonging to the immunoglobulin superfamily, containing a single Ig-like V-type domain.
• It is primarily expressed on the cell membrane, where it may mediate cell-cell and cell-ligand interactions, particularly in neuronal tissues.
• Functional studies suggest a role in synaptogenesis and other brain-related events, though its precise molecular mechanisms remain under investigation.
• Post-translational modifications include N-linked glycosylation, disulfide bond formation, and a GPI anchor that tethers it to the outer leaflet of the plasma membrane.
• The protein is synthesized with a signal peptide (amino acids 1-19) and the mature form spans residues 20-130, which corresponds to the immunogen used for this antibody.
• Keywords associated with CD90/Thy-1 in UniProtKB include cell membrane, GPI-anchor, glycoprotein, immunoglobulin domain, lipoprotein, phosphoprotein, and signal peptide.
• The predicted molecular weight of the unprocessed precursor is approximately 18 kDa, consistent with the supplier-reported protein weight.

Experimental Guidance and Technical Tips

• This antibody targets the extracellular domain (amino acids 20-130) of human CD90/Thy-1 and is directly conjugated to PE/Cyanine7, making it suitable for direct flow cytometric staining without secondary reagents.
• For flow cytometry, it is recommended to titrate the antibody to determine the optimal dilution for your specific cell type and experimental conditions, and to include appropriate isotype controls.
• CD90 is widely expressed on various stem and progenitor cells, as well as in neuronal tissues; therefore, validate expression in the relevant cell population before analysis.
• Since the antibody is provided as a liquid conjugate, protect from light and avoid freeze-thaw cycles to maintain fluorophore stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic and life science manufacturers by providing ready-to-use, validated raw materials like this anti-CD90 monoclonal antibody, along with complementary antibody pairs and bulk ancillary reagents. For researchers delving into stem cell biology, neurobiology, or immune profiling, CamelBio streamlines access to rare-target antibodies and offers technical consultation to bridge the gap from concept to clinical application. Whether you need custom formulations or off-the-shelf solutions, we serve as a dedicated sourcing partner.