PE/Cyanine7 Rabbit anti-Human CD8b Monoclonal Antibody for FC - P10966

Core Product Specifications and Parameters

Biological Background: CD8 Beta Chain Function and Localization

• CD8B encodes the beta chain of the CD8 glycoprotein, a T-cell surface receptor that exists as multiple isoforms (isoforms 1, 3, 5, 6, 7, 8) due to alternative splicing. Isoform 1 is predominantly expressed in thymus, while isoform 6 is more abundant in peripheral CD8+ T-cells.
• The protein functions as a coreceptor for MHC class I molecules on antigen-presenting cells, binding simultaneously with the T-cell receptor (TCR) to enhance T-cell activation.
• The cytoplasmic tail contains a palmitoylation site that targets CD8 to lipid rafts, facilitating efficient signal transduction.
• Upon MHC I engagement, CD8B recruits the Src kinase LCK to the TCR-CD3 complex, initiating phosphorylation cascades that lead to lymphokine production, motility, adhesion, and cytotoxic T-lymphocyte (CTL) activation.
• Plays a critical role in thymic selection of CD8+ T-cells during development.
• The protein is a transmembrane glycoprotein with disulfide bonds and belongs to the immunoglobulin superfamily.
• Primarily localized to the cell membrane, but secreted isoforms also exist.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 22-170) of human CD8b, so the antibody likely recognizes native conformation on live cells, making it suitable for flow cytometry.
• For FC, titrate the antibody to determine optimal staining concentration for your cell type. Use appropriate controls (isotype control, unstained cells) to assess specificity.
• Protect the PE/Cyanine7 conjugate from light to maintain fluorescence stability.
• Consider fixing cells after staining if necessary, but note that fixation may affect epitope accessibility.
• Validate cross-reactivity in human samples; for other species, confirm sequence homology beforehand.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers and research labs, offering a wide range of IVD raw materials. This PE/Cyanine7 anti-human CD8b monoclonal antibody is part of our portfolio for immune monitoring and T-cell research applications. We provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to support your assay development from concept to clinic.