PE/Cyanine7 Rabbit anti-Human CD42b Monoclonal Antibody for FC - P07359

Core Product Specifications and Parameters

Biological Background: CD42b Function and Localization

• Encoded by the GP1BA gene, CD42b (platelet glycoprotein Ib alpha chain, also known as GPIbA, CD42b-alpha) is a 72 kDa type I transmembrane protein expressed on the surface of platelets.
• CD42b is a key component of the GPIb-V-IX complex, which mediates platelet adhesion to the subendothelium by binding von Willebrand factor (vWF), initiating primary hemostasis and thrombus formation.
• The extracellular domain features leucine-rich repeats critical for vWF binding, while the intracellular domain interacts with the cytoskeleton, facilitating platelet activation and shape change.
• Located on the plasma membrane, CD42b functions as a receptor that detects vascular injury, responding to exposed subendothelial matrix via immobilized vWF A1 domain.
• Mutations in GP1BA are linked to inherited bleeding disorders, including Bernard-Soulier syndrome (giant platelet disorder) and platelet-type von Willebrand disease, underscoring its essential role in hemostasis.
• Post-translational modifications include tyrosine sulfation and phosphorylation, which may regulate receptor function and signal transduction.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (amino acids 15–531) of human CD42b, suggesting that the antibody likely targets an epitope accessible on live, unpermeabilized cells.
• Designed for flow cytometry, consider optimizing antibody dilution and using an appropriate isotype control to distinguish specific binding, especially in platelet-rich plasma or whole blood samples.
• For platelet staining, use gentle handling and avoid EDTA-based anticoagulants if they affect GPIb alpha integrity; sodium citrate or heparin may be considered.
• This antibody may be suitable for analyzing CD42b expression in studies of platelet function, thrombocytopenia, or GP1BA-related disorders; confirm specificity by comparing with a known positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with integrated access to high-quality IVD raw materials, including a broad portfolio of validated monoclonal antibodies like this CD42b clone. Our one-stop platform streamlines procurement of platelet biology reagents, bundled with technical support for flow cytometry assay development. Whether you need optimized antibody pairs, bulk ancillary reagents, or custom sourcing for rare hemostasis targets, CamelBio serves as your reliable bridge from concept to clinic.