PE/Cyanine7 Rabbit anti-Human CD38 mAb for FC - P28907

Core Product Specifications and Parameters

Biological Background: CD38 Function and Localization

• Encoded by the CD38 gene, the canonical CD38 protein (also known as ADPRC1, cADPR1) is a ~34 kDa type II transmembrane glycoprotein with enzymatic and receptor functions. Alternative splicing generates isoforms, including a 13 kDa variant.
• Acts as an ADP-ribosyl cyclase to produce cyclic ADP-ribose (cADPR), a potent second messenger that mobilizes calcium and regulates glucose-induced insulin secretion, and also exhibits cADPR hydrolase activity. Related references: PMID:7961800, PMID:8253715
• Synthesizes nicotinate-adenine dinucleotide phosphate (NAADP+), another calcium-mobilizing second messenger, from 2′-phospho-cADPR or NADP+ and nicotinic acid, preferentially at acidic pH. Related references: PMID:16690024
• Functions as a cell-surface receptor for CD31 (PECAM-1) on endothelial cells, facilitating lymphocyte adhesion, activation, proliferation, and transendothelial migration. Related references: PMID:9551996
• In lamina propria T lymphocytes, CD38/CD31 engagement triggers a signaling cascade involving LCK and LAT phosphorylation, leading to cytokine production. Related references: PMID:11259373
• Regulates mature dendritic cell functions essential for adaptive immunity, including CCL21-mediated chemotaxis, cell survival, and Th1 polarization. Related references: PMID:16293598
• Primarily localized to the cell surface and membrane; highly expressed in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma, and neuroblastoma.
• Post-translational modifications include disulfide bond formation and N-linked glycosylation, which are critical for proper folding and enzymatic activity.

Experimental Guidance and Technical Tips

• This PE/Cyanine7-conjugated monoclonal antibody is optimized for direct flow cytometry (FC) without the need for a secondary antibody. Use with appropriate isotype control (e.g., rabbit IgG PE/Cyanine7) to assess background.
• Since CD38 is a cell surface protein, stain viable, unfixed cells to preserve epitope integrity. For intracellular detection, validate fixation/permeabilization protocols that do not compromise the extracellular domain epitope.
• The immunogen (extracellular domain residues 43–300) ensures recognition of the native conformation; confirm reactivity in your target cell system using known CD38-expressing cells (e.g., lymphocytes, plasma cells) as positive controls.
• For quantitative FC, titrate the antibody to determine optimal staining concentration and avoid non-specific binding. Lyse red blood cells and use Fc block when working with whole blood.
• Expected molecular weights: 34 kDa for the canonical isoform, ~13 kDa for a short alternatively spliced variant; this may be useful if employing complementary methods like western blotting with a validated anti-CD38 primary antibody.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and ancillary reagents. This PE/Cyanine7 anti-CD38 monoclonal antibody supports flow-cytometry-based immune profiling. CamelBio can support your development with bulk quantities, customized conjugates, and sourcing of rare-target raw materials.